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Luceri F, Balboni F, Balato G, et al. Analytical Evaluation of a Vancomycin Immunoassay in Synovial Fluid. Joints. 2017;5(2):125-126doi: 10.1055/s-0037-1603671.
Luceri, F., Balboni, F., Balato, G., Pezzati, P., Cini, N., Virgili, G., & Baldini, A. (2017). Analytical Evaluation of a Vancomycin Immunoassay in Synovial Fluid. Joints, 5(2), 125-126. https://doi.org/10.1055/s-0037-1603671
Luceri, Francesca, et al. "Analytical Evaluation of a Vancomycin Immunoassay in Synovial Fluid." Joints vol. 5,2 (2017): 125-126. doi: https://doi.org/10.1055/s-0037-1603671
Luceri F, Balboni F, Balato G, Pezzati P, Cini N, Virgili G, Baldini A. Analytical Evaluation of a Vancomycin Immunoassay in Synovial Fluid. Joints. 2017 Jul 28;5(2):125-126. doi: 10.1055/s-0037-1603671. eCollection 2017 Jun. PMID: 29114643; PMCID: PMC5672864.
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Joints. 2017 Jul 28;5(2):125-126. doi: 10.1055/s-0037-1603671. eCollection 2017 Jun.

Analytical Evaluation of a Vancomycin Immunoassay in Synovial Fluid.

Joints

Francesca Luceri, Fiamma Balboni, Giovanni Balato, Paola Pezzati, Nicoletta Cini, Gianni Virgili, Andrea Baldini

Affiliations

  1. Laboratorio Generale, Dipartimento dei Servizi, Azienda Ospedaliero Universitaria Careggi, Firenze, Italy.
  2. Laboratorio Analisi, Istituto Fiorentino Cura E Assistenza, Firenze, Italy.
  3. UO Ortopedia, Istituto Fiorentino Cura E Assistenza, Firenze, Italy.
  4. Dipartimento di Chirurgia e Medicina Translazionale, Università di Firenze, Oculistica, Italy.

PMID: 29114643 PMCID: PMC5672864 DOI: 10.1055/s-0037-1603671

Abstract

In clinical laboratories performing routine activities, the need to answer the burning clinical question in emerging field may be limited by lack of technology support or assays accessibility. Commercially available methods, although originally validated for specific biological matrices, may be employed for other matrices, following appropriate guidelines such as Clinical and Laboratory Standards Institute (CLSI) EP 19. We investigated the use of a vancomycin assay with synovial fluid samples, in view of a possible employment in vancomycin release study. The standard of care of periprosthetic joint infection is a two-stage revision surgery with antibiotic-loaded bone cement implantation. Vancomycin, for its activity against gram-positive bacteria even multidrug-resistant staphylococci, is the most widely used antibiotic. Despite the widespread use of such devices, little is known about the in vivo elution in the joint space. Clinical laboratories equipped with a validated, affordable method to quantify vancomycin in synovial fluid, may support clinical research, and give an important contribution to the study of the pharmacokinetics of antibiotic release from bone cement matrix.

Keywords: antibacterial agents; clinical laboratory services; immunoassay synovial fluid; vancomycin

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