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Sarnicola V, Millacci C, Sarnicola E, et al. Suture pull-through insertion of graft donor in Descemet stripping automated endothelial keratoplasty: Results of 4-year follow-up. Taiwan J Ophthalmol. 2015;5(3):114-119doi: 10.1016/j.tjo.2015.07.001.
Sarnicola, V., Millacci, C., Sarnicola, E., Sarnicola, C., Sabatino, F., & Ruggiero, A. (2015). Suture pull-through insertion of graft donor in Descemet stripping automated endothelial keratoplasty: Results of 4-year follow-up. Taiwan journal of ophthalmology, 5(3), 114-119. https://doi.org/10.1016/j.tjo.2015.07.001
Sarnicola, Vincenzo, et al. "Suture pull-through insertion of graft donor in Descemet stripping automated endothelial keratoplasty: Results of 4-year follow-up." Taiwan journal of ophthalmology vol. 5,3 (2015): 114-119. doi: https://doi.org/10.1016/j.tjo.2015.07.001
Sarnicola V, Millacci C, Sarnicola E, Sarnicola C, Sabatino F, Ruggiero A. Suture pull-through insertion of graft donor in Descemet stripping automated endothelial keratoplasty: Results of 4-year follow-up. Taiwan J Ophthalmol. 2015 Jul-Sep;5(3):114-119. doi: 10.1016/j.tjo.2015.07.001. Epub 2015 Aug 28. PMID: 29018681; PMCID: PMC5602706.
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Taiwan J Ophthalmol. 2015 Jul-Sep;5(3):114-119. doi: 10.1016/j.tjo.2015.07.001. Epub 2015 Aug 28.

Suture pull-through insertion of graft donor in Descemet stripping automated endothelial keratoplasty: Results of 4-year follow-up.

Taiwan journal of ophthalmology

Vincenzo Sarnicola, Chiara Millacci, Enrica Sarnicola, Caterina Sarnicola, Francesco Sabatino, Andrew Ruggiero

Affiliations

  1. Ambulatorio di Chirurgia Oculare Santa Lucia, Grosseto, Italy.
  2. Department of Ophthalmology, Misericordia Hospital, Grosseto, Italy.
  3. Department of Ophthalmology, Università degli Studi di Siena, Siena, Italy.
  4. Department of Ophthalmology, Università degli Studi di Ferrara, Ferrara, Italy.
  5. Department of Ophthalmology, Università Campus Bio-Medico di Roma, Rome, Italy.
  6. Department of Civil and Mechanical Engineering, DiMSAT, Università di Cassino, Cassino, Italy.

PMID: 29018681 PMCID: PMC5602706 DOI: 10.1016/j.tjo.2015.07.001

Abstract

PURPOSE: To report our clinical experience and 4-year follow-up results of Descemet stripping automated endothelial keratoplasty (DSAEK) with the suture pull-through insertion technique.

METHODS: This is a retrospective study of 195 eyes in which a posterior lamellar keratoplasty was performed between 2007 and 2011. The insertion of a folded donor lenticule was performed with a double-armed 10-0 suture using a straight transchamber needle and half-circle needle. Endothelial cell density was measured annually up to 4 years after the surgery, and cell loss was calculated based on the median preoperative donor endothelial cell density. Postoperative complications, primary graft failure, pupillary block, and dislocation of the donor tissue were assessed.

RESULTS: All patients underwent uncomplicated DSAEK. Data were available for 195 eyes (100%) at 1 year, 186 eyes (95.3%) at 2 years, 176 eyes (90.2%) at 3 years, and 160 eyes (82%) at 4 years. Median preop-erative donor endothelial cell density was 2688 cells/mm

CONCLUSION: DSAEK with suture pull-through insertion of donor graft represents a simplified and safe technique that has endothelial cell loss comparable with other techniques and low rates of intraoperative and postoperative complications.

Keywords: Descemet stripping automated endothelial keratoplasty; endothelial cell loss; suture pull-through donor insertion

Conflict of interest statement

Conflicts of interest: All contributing authors declare no conflicts of interest.

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