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Katz-Summercorn A, Anand S, Ingledew S, et al. Application of a multi-gene next-generation sequencing panel to a non-invasive oesophageal cell-sampling device to diagnose dysplastic Barrett's oesophagus. J Pathol Clin Res. 2017;3(4):258-267doi: 10.1002/cjp2.80.
Katz-Summercorn, A., Anand, S., Ingledew, S., Huang, Y., Roberts, T., Galeano-Dalmau, N., O'Donovan, M., Liu, H., & Fitzgerald, R. C. (2017). Application of a multi-gene next-generation sequencing panel to a non-invasive oesophageal cell-sampling device to diagnose dysplastic Barrett's oesophagus. The journal of pathology. Clinical research, 3(4), 258-267. https://doi.org/10.1002/cjp2.80
Katz-Summercorn, Annalise, et al. "Application of a multi-gene next-generation sequencing panel to a non-invasive oesophageal cell-sampling device to diagnose dysplastic Barrett's oesophagus." The journal of pathology. Clinical research vol. 3,4 (2017): 258-267. doi: https://doi.org/10.1002/cjp2.80
Katz-Summercorn A, Anand S, Ingledew S, Huang Y, Roberts T, Galeano-Dalmau N, O'Donovan M, Liu H, Fitzgerald RC. Application of a multi-gene next-generation sequencing panel to a non-invasive oesophageal cell-sampling device to diagnose dysplastic Barrett's oesophagus. J Pathol Clin Res. 2017 Aug 24;3(4):258-267. doi: 10.1002/cjp2.80. eCollection 2017 Oct. PMID: 29085666; PMCID: PMC5653927.
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J Pathol Clin Res. 2017 Aug 24;3(4):258-267. doi: 10.1002/cjp2.80. eCollection 2017 Oct.

Application of a multi-gene next-generation sequencing panel to a non-invasive oesophageal cell-sampling device to diagnose dysplastic Barrett's oesophagus.

The journal of pathology. Clinical research

Annalise Katz-Summercorn, Shubha Anand, Sophie Ingledew, Yuanxue Huang, Thomas Roberts, Nuria Galeano-Dalmau, Maria O'Donovan, Hongxiang Liu, Rebecca C Fitzgerald

Affiliations

  1. MRC Cancer Unit, University of Cambridge, Hutchison/MRC Research CentreBox 197, Cambridge Biomedical CampusCambridgeUK.
  2. Molecular Malignancy Laboratory, Haematology and Oncology Diagnostic ServiceAddenbrooke's Hospital, Cambridge University Hospitals NHS Foundation TrustCambridgeUK.

PMID: 29085666 PMCID: PMC5653927 DOI: 10.1002/cjp2.80

Abstract

The early detection and endoscopic treatment of patients with the dysplastic stage of Barrett's oesophagus is a key to preventing progression to oesophageal adenocarcinoma. However, endoscopic surveillance protocols are hampered by the invasiveness of repeat endoscopy, sampling bias, and a subjective histopathological diagnosis of dysplasia. In this case-control study, we investigated the use of a non-invasive, pan-oesophageal cell-sampling device, the Cytosponge™, coupled with a cancer hot-spot panel to identify patients with dysplastic Barrett's oesophagus. Formalin-fixed, paraffin-embedded (FFPE) Cytosponge™ samples from 31 patients with non-dysplastic and 28 with dysplastic Barrett's oesophagus with good available clinical annotation were selected for inclusion. Samples were microdissected and amplicon sequencing performed using a panel covering

Keywords: Barrett's oesophagus; Cytosponge™; biomarker; dysplasia; hot‐spot panel; oesophageal adenocarcinoma

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