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Jenvey CJ, Reichel MP, Lanyon SR, et al. Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves. Vet Sci. 2015;2(1):26-31doi: 10.3390/vetsci2010026.
Jenvey, C. J., Reichel, M. P., Lanyon, S. R., & Cockcroft, P. D. (2015). Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves. Veterinary sciences, 2(1), 26-31. https://doi.org/10.3390/vetsci2010026
Jenvey, Caitlin J, et al. "Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves." Veterinary sciences vol. 2,1 (2015): 26-31. doi: https://doi.org/10.3390/vetsci2010026
Jenvey CJ, Reichel MP, Lanyon SR, Cockcroft PD. Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves. Vet Sci. 2015 Mar 09;2(1):26-31. doi: 10.3390/vetsci2010026. PMID: 29061928; PMCID: PMC5644607.
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Vet Sci. 2015 Mar 09;2(1):26-31. doi: 10.3390/vetsci2010026.

Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves.

Veterinary sciences

Caitlin J Jenvey, Michael P Reichel, Sasha R Lanyon, Peter D Cockcroft

Affiliations

  1. School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia. [email protected].
  2. School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia. [email protected].
  3. School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia. [email protected].
  4. School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia. [email protected].

PMID: 29061928 PMCID: PMC5644607 DOI: 10.3390/vetsci2010026

Abstract

Colostrum contains substantially higher concentrations of immunoglobulins compared to serum, which may help to improve the utility of diagnostic tests. The aim of this study was to determine the diagnostic value of colostrum antibody concentrations in identifying Bovine Viral Diarrhoea Virus (BVDV) PI (persistently infected) calf carrying beef heifers following an experimental infection. Colostrum was collected within 12 hours of parturition and tested in undiluted, 1:5, 1:10, 1:100, 1:200, and 1:500 dilutions using an enzyme-linked immunosorbent assay (ELISA) for BVDV antibody. Cows were determined to be carrying a PI calf based on positive quantitative Real Time-Polymerase Chain Reaction and antigen ELISA result on pre-colostral serum and ear notch samples collected from their calf. The median ELISA sample-to-positive (S/P) ratio for colostrum collected from heifers that carried a PI calf were significantly higher than the median ELISA S/P ratio for colostrum collected from heifers that did not carry a PI calf at dilutions of 1:100, 1:200, and 1:500. This study provides further evidence for increased antigenic stimulation

Keywords: BVDV; ELISA; PI calves; colostrum

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