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Seifert GJ, Sander KC, Richter S, et al. Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study. Ann Med Surg (Lond). 2017;24:8-14doi: 10.1016/j.amsu.2017.09.012.
Seifert, G. J., Sander, K. C., Richter, S., Wittel, U. A., Greene, D., Hoffmann, A. L., & Stark, L. (2017). Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study. Annals of medicine and surgery (2012), 248-14. https://doi.org/10.1016/j.amsu.2017.09.012
Seifert, Gabriel J, et al. "Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study." Annals of medicine and surgery (2012) vol. 24 (2017): 8-14. doi: https://doi.org/10.1016/j.amsu.2017.09.012
Seifert GJ, Sander KC, Richter S, Wittel UA, Greene D, Hoffmann AL, Stark L. Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study. Ann Med Surg (Lond). 2017 Sep 28;24:8-14. doi: 10.1016/j.amsu.2017.09.012. eCollection 2017 Dec. PMID: 29062479; PMCID: PMC5645008.
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Ann Med Surg (Lond). 2017 Sep 28;24:8-14. doi: 10.1016/j.amsu.2017.09.012. eCollection 2017 Dec.

Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study.

Annals of medicine and surgery (2012)

Gabriel J Seifert, Karoline C Sander, Sabine Richter, Uwe A Wittel, Greene, Hoffmann, Stark

Affiliations

  1. Department of General and Visceral Surgery, Medical Center - University of Freiburg, Freiburg, Germany.

PMID: 29062479 PMCID: PMC5645008 DOI: 10.1016/j.amsu.2017.09.012

Abstract

BACKGROUND: Little is known regarding the impact of host response in acute pancreatitis. Here, we induce murine necrotizing pancreatitis in 9 different mouse strains.

MATERIALS AND METHODS: We examined 9 different mouse strains: Balb/CB4J, C3H/HEJ, NOD/SHILT, A/J, AKR/J, C57BI/6J, DBA/2J, FVB/NJ, 129S1/SvlmJ. 10 animals per strain were randomly allotted to two groups. Sterile necrotizing pancreatitis was induced by injection of taurocholate into the common bile duct. Control animals were injected with saline. Every 6 h, clinical parameters were examined and scored. After 24 h, animals were sacrificed to examine and compare serum enzymes, histology, bronchoalveolar lavage fluid, and serum IL-6.

RESULTS: Histologically, taurocholate treated animals scored significantly higher than control animals. Concordantly, serum lipase and amylase were significantly elevated in pancreatitis animals in all strains. NOD/SHILT and AKR/J mice had the highest enzyme activity. 24 h after induction, there were no signs of increased pulmonary vascular leak in taurocholate animals. Remarkably, interleukin 6 was not increased at all in C57BL/6J, C3H/HeJ, and 129S1/SvlmJ mice compared to all other strains.

CONCLUSION: The genetic strain has an impact on pancreatitis severity and systemic inflammatory response in a murine taurocholate induction model. Analogous differences in humans may partially account for the disparity in post-ERCP pancreatitis.

Keywords: Genetic background; Genetic strain; Murine pancreatitis; Severe acute pancreatitis; Taurocholate

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