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Ali F, Aziz F, Wajid N. Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells . Chronic Dis Transl Med. 2017;3(2):105-111doi: 10.1016/j.cdtm.2017.02.006.
Ali, F., Aziz, F., & Wajid, N. (2017). Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells . Chronic diseases and translational medicine, 3(2), 105-111. https://doi.org/10.1016/j.cdtm.2017.02.006
Ali, Fatima, et al. "Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells ." Chronic diseases and translational medicine vol. 3,2 (2017): 105-111. doi: https://doi.org/10.1016/j.cdtm.2017.02.006
Ali F, Aziz F, Wajid N. Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells . Chronic Dis Transl Med. 2017 Apr 17;3(2):105-111. doi: 10.1016/j.cdtm.2017.02.006. eCollection 2017 Jun 25. PMID: 29063063; PMCID: PMC5627692.
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Chronic Dis Transl Med. 2017 Apr 17;3(2):105-111. doi: 10.1016/j.cdtm.2017.02.006. eCollection 2017 Jun 25.

Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells .

Chronic diseases and translational medicine

Fatima Ali, Fehmina Aziz, Nadia Wajid

Affiliations

  1. Institute of Molecular Biology and Biotechnology (IMBB) & Centre for Research In Molecular Medicine (CRIMM), The University of Lahore, Raiwind Road, Lahore, Pakistan.

PMID: 29063063 PMCID: PMC5627692 DOI: 10.1016/j.cdtm.2017.02.006

Abstract

OBJECTIVE: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior.

METHODS: WJMSCs at passage 3 were treated with serum derived from type 2 diabetic patients. WJMSCs were characterized for surface markers expression by using immunocytochemistry technique. The effects on cell viability, proliferation, cell death rate, and vascular endothelial growth factor level were assessed by crystal violet staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase assay, and enzyme-linked immuno-sorbent assay, respectively. Oxidative stress was assessed by the estimation of free radical species malondialdehyde (MDA) and enzymes glutathione (GSH), catalase, and superoxide dismutase (SOD).

RESULTS: WJMSCs isolated in this study were positive for CD29, CD49, CD73, CD90, CD105, and SSEA4 and negative for CD45 and CD34. Under diabetic stress conditions, WJMSCs showed low viability and high lactate dehydrogenase release. A low level of vascular endothelial growth factor was also observed after diabetic serum treatment. Antioxidant level was also lower in diabetic serum-treated WJMSCs compared to in normal serum-treated WJMSCs.

CONCLUSION: The results of the present study suggest that pre-treatment of WJMSCs with type 2 diabetic serum decreases the survival of WJMSCs. The findings of this study provide insight into diabetes-induced harmful effects on WJMSCs.

Keywords: Oxidative stress; Type 2 diabetes mellitus; Vascular endothelial growth factor; Wharton jelly-derived mesenchymal stem cells

References

  1. J Biochem Mol Toxicol. 2003;17(1):24-38 - PubMed
  2. Curr Pharm Des. 2013;19(32):5695-703 - PubMed
  3. Cell Tissue Bank. 2015 Sep;16(3):389-97 - PubMed
  4. Stem Cells Int. 2015;2015:583984 - PubMed
  5. Indian Heart J. 2016 May-Jun;68(3):325-31 - PubMed
  6. Cells Tissues Organs. 2013;198(3):198-208 - PubMed
  7. Circ Res. 2010 Oct 29;107(9):1058-70 - PubMed
  8. Diabetes. 2014 Feb;63(2):675-87 - PubMed
  9. Stem Cell Res Ther. 2013 May 24;4(3):58 - PubMed
  10. Biomed Res Int. 2015;2015:430847 - PubMed
  11. Int J Mol Sci. 2013 May 31;14(6):11692-712 - PubMed
  12. Diabetes. 2012 Mar;61(3):728-38 - PubMed
  13. J Immunol. 2010 Dec 1;185(11):6617-23 - PubMed
  14. Diabetes Care. 2010 Jan;33 Suppl 1:S11-61 - PubMed
  15. Exp Diabetes Res. 2012;2012:696215 - PubMed

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