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Chen KH, Yang CH, Wallace CG, et al. Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat. Am J Transl Res. 2017;9(10):4593-4606
Chen, K. H., Yang, C. H., Wallace, C. G., Lin, C. R., Liu, C. K., Yin, T. C., Huang, T. H., Chen, Y. L., Sun, C. K., & Yip, H. K. (2017). Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat. American journal of translational research, 9(10), 4593-4606.
Chen, Kuan-Hung, et al. "Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat." American journal of translational research vol. 9,10 (2017): 4593-4606.
Chen KH, Yang CH, Wallace CG, Lin CR, Liu CK, Yin TC, Huang TH, Chen YL, Sun CK, Yip HK. Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat. Am J Transl Res. 2017 Oct 15;9(10):4593-4606. eCollection 2017. PMID: 29118920; PMCID: PMC5666067.
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Am J Transl Res. 2017 Oct 15;9(10):4593-4606. eCollection 2017.

Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat.

American journal of translational research

Kuan-Hung Chen, Chien-Hui Yang, Christopher Glenn Wallace, Chung-Ren Lin, Chia-Kai Liu, Tsung-Cheng Yin, Tien-Hung Huang, Yi-Ling Chen, Cheuk-Kwan Sun, Hon-Kan Yip

Affiliations

  1. Department of Anesthesiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301, Taiwan, R.O.C.
  2. Department of Plastic Surgery, University Hospital of South ManchesterManchester, UK.
  3. Department of Orthopedics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301, Taiwan, R.O.C.
  4. Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301, Taiwan, R.O.C.
  5. Department of Emergency Medicine, E-Da Hospital, I-Shou University School of Medicine for International StudentsKaohsiung 82445, Taiwan, R.O.C.
  6. Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301, Taiwan, R.O.C.
  7. Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301, Taiwan, R.O.C.
  8. Department of Medical Research, China Medical University Hospital, China Medical UniversityTaichung 40402, Taiwan, R.O.C.
  9. Department of Nursing, Asia UniversityTaichung 41354, Taiwan, R.O.C.

PMID: 29118920 PMCID: PMC5666067

Abstract

This study tested the hypothesis that combination therapy using extracorporeal shock wave (ECSW)-melatonin (Mel) was superior to either alone at ameliorating neuropathic pain (NP). NP was induced by chronic constriction injury (CCI) to the left sciatic nerve in rats. Animals were categorized into sham control (group 1), CCI only (group 2), CCI-ECSW (group 3), CCI-Mel (group 4) and CCI-ECSW-Mel (group 5). By days 2 and 8 after CCI, the mechanical paw withdrawal threshold (MPWT)/thermal paw withdrawal latency (TPWL) were highest in group 2, lowest in group 1, significantly lower in group 5 than in groups 3 and 4 (all p<0.0001), and not significantly different between groups 3 and 4. The protein expressions of inflammatory (TNF-α/NF-κB/MMP-9/IL-1ß/GFAP/ox42), oxidative-stress (NOX-1/NOX-2/NOX-4/oxidized protein), DNA/mitochondrial-damaged (γ-H2AX/cytosolic mitochondria), apoptotic (cleaved capase-3/PARP), and MAPK family biomarkers (p-P38/p-JNK/p-ERK1/2) in dorsal root ganglia and spinal dorsal horn expressed a similar pattern of MPWT/TPWL among the five groups, except for significantly higher in group 4 than in group 3 (all p<0.0001). The protein expressions of Nav.1.3, Nav.1.8 and Nav.1.9 in sciatic nerve displayed an identical pattern to inflammation among the five groups (all p<0.001). Pain facilitated cellular expressions (p-P38+/peripherin+ cells, P38+/NF200+ cells) displayed an identical pattern to inflammation among the five groups (all p<0.0001). In conclusion, ECSW-Mel combination therapy markedly ameliorated NP induced by CCI.

Keywords: Neuropathic pain; chronic constriction injury; extracorporeal shock wave; inflammation; melatonin; oxidative stress

Conflict of interest statement

None.

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