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Chung JR, Choi JW, Fiorellini JP, et al. Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration. J Dent Anesth Pain Med. 2017;17(3):191-198doi: 10.17245/jdapm.2017.17.3.191.
Chung, J. R., Choi, J. W., Fiorellini, J. P., Hwang, K. G., & Park, C. J. (2017). Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration. Journal of dental anesthesia and pain medicine, 17(3), 191-198. https://doi.org/10.17245/jdapm.2017.17.3.191
Chung, Joo-Ryun, et al. "Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration." Journal of dental anesthesia and pain medicine vol. 17,3 (2017): 191-198. doi: https://doi.org/10.17245/jdapm.2017.17.3.191
Chung JR, Choi JW, Fiorellini JP, Hwang KG, Park CJ. Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration. J Dent Anesth Pain Med. 2017 Sep;17(3):191-198. doi: 10.17245/jdapm.2017.17.3.191. Epub 2017 Sep 25. PMID: 29090249; PMCID: PMC5647825.
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J Dent Anesth Pain Med. 2017 Sep;17(3):191-198. doi: 10.17245/jdapm.2017.17.3.191. Epub 2017 Sep 25.

Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration.

Journal of dental anesthesia and pain medicine

Joo-Ryun Chung, Jong-Won Choi, Joseph P Fiorellini, Kyung-Gyun Hwang, Chang-Joo Park

Affiliations

  1. Division of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Hanyang University, Seoul, Korea.
  2. Department of Periodontics, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA.

PMID: 29090249 PMCID: PMC5647825 DOI: 10.17245/jdapm.2017.17.3.191

Abstract

BACKGROUND: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds.

METHODS: In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated.

RESULTS: The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF).

CONCLUSIONS: Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits.

Keywords: Cellular Attachment; Fibronectins; Nerve Cell; Nerve Growth Factor; Tenascin

Conflict of interest statement

CONFLICTS OF INTEREST: The authors report no conflicts of interest.

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