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Gómez-Sintes R, Villarejo-Zori B, Serrano-Puebla A, et al. Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells. 2017;6(4)doi: 10.3390/cells6040037.
Gómez-Sintes, R., Villarejo-Zori, B., Serrano-Puebla, A., Esteban-Martínez, L., Sierra-Filardi, E., Ramírez-Pardo, I., Rodríguez-Muela, N., & Boya, P. (2017). Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells, 6(4), . https://doi.org/10.3390/cells6040037
Gómez-Sintes, Raquel, et al. "Standard Assays for the Study of Autophagy in the Ex Vivo Retina." Cells vol. 6,4 (2017). doi: https://doi.org/10.3390/cells6040037
Gómez-Sintes R, Villarejo-Zori B, Serrano-Puebla A, Esteban-Martínez L, Sierra-Filardi E, Ramírez-Pardo I, Rodríguez-Muela N, Boya P. Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells. 2017 Oct 22;6(4). doi: 10.3390/cells6040037. PMID: 29065501; PMCID: PMC5755496.
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Cells. 2017 Oct 22;6(4). doi: 10.3390/cells6040037.

Standard Assays for the Study of Autophagy in the Ex Vivo Retina.

Cells

Raquel Gómez-Sintes, Beatriz Villarejo-Zori, Ana Serrano-Puebla, Lorena Esteban-Martínez, Elena Sierra-Filardi, Ignacio Ramírez-Pardo, Natalia Rodríguez-Muela, Patricia Boya

Affiliations

  1. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  2. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  3. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  4. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  5. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  6. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  7. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].
  8. Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain. [email protected].

PMID: 29065501 PMCID: PMC5755496 DOI: 10.3390/cells6040037

Abstract

Autophagy is a catabolic pathway that mediates the degradation and recycling of intracellular components, and is a key player in a variety of physiological processes in cells and tissues. Recent studies of autophagy in the eye suggest that this pathway is fundamental for the preservation of retinal homeostasis. Given its accessible location outside the brain, the retina is an ideal organ in which to study the central nervous system and a wide range of neuronal processes, from development to neurodegeneration. Here we review several methods used to assess autophagy in the retina in both physiological and pathological conditions.

Keywords: autophagy; lysosomes; mitophagy; organotypic cultures; retina

Conflict of interest statement

The authors declare no conflicts of interest.

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