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Adams DL, Alpaugh RK, Martin SS, et al. Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells. RSC Adv. 2016;6(8):6405-6414doi: 10.1039/c5ra21524b.
Adams, D. L., Alpaugh, R. K., Martin, S. S., Charpentier, M., Chumsri, S., Cristofanilli, M., Adams, D. K., Makarova, O. V., Zhu, P., Li, S., Tang, C. M., & Stefansson, S. (2016). Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells. RSC advances, 6(8), 6405-6414. https://doi.org/10.1039/c5ra21524b
Adams, Daniel L, et al. "Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells." RSC advances vol. 6,8 (2016): 6405-6414. doi: https://doi.org/10.1039/c5ra21524b
Adams DL, Alpaugh RK, Martin SS, Charpentier M, Chumsri S, Cristofanilli M, Adams DK, Makarova OV, Zhu P, Li S, Tang CM, Stefansson S. Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells. RSC Adv. 2016;6(8):6405-6414. doi: 10.1039/c5ra21524b. Epub 2016 Jan 11. PMID: 29093811; PMCID: PMC5662211.
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RSC Adv. 2016;6(8):6405-6414. doi: 10.1039/c5ra21524b. Epub 2016 Jan 11.

Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells.

RSC advances

Daniel L Adams, R Katherine Alpaugh, Stuart S Martin, Monica Charpentier, Saranya Chumsri, Massimo Cristofanilli, Diane K Adams, Olga V Makarova, Peixuan Zhu, Shuhong Li, Cha-Mei Tang, Steingrimur Stefansson

Affiliations

  1. Creatv MicroTech, Inc., 1 Deer Park Dr., Monmouth Junction, NJ 08852.
  2. Fox Chase Cancer Center, Protocol Support Laboratory, 333 Cottman Ave., Philadelphia, PA 19111.
  3. University of Maryland Baltimore Greenebaum Cancer Center, 655 W. Baltimore St., Baltimore, MD 21136.
  4. Mayo Clinic Cancer Center, 4500 San Pablo Rd., Jacksonville, FL 32224.
  5. Robert H Lurie Comprehensive Cancer Center, Northwestern University, 645 N Michigan Avenue, Chicago, IL 60611.
  6. Rutgers, the State University of New Jersey, 71 Dudley Rd., New Brunswick, NJ 08901.
  7. Creatv MicroTech, Inc., 2242 West Harrison St., Chicago IL, 60612.
  8. Creatv MicroTech, Inc., 9900 Belward Campus Dr. Rockville, MD 20850.
  9. Hememics Biotechnologies, 12111 Parklawn Dr. Rockville MD, 20852.

PMID: 29093811 PMCID: PMC5662211 DOI: 10.1039/c5ra21524b

Abstract

Enumeration of circulating tumor cells (CTCs) from cancer patient blood is an established diagnostic assay used to evaluate patient status as a singleplex test. However, in the coming age of personalized medicine, multiplex analysis of patient CTCs, including proteomic and genomic techniques, will have to be integrated with CTC isolation platform technologies. Advancements in microfabrication have demonstrated that CTCs can be isolated and analyzed using microfluidic lab-on-a-chip devices. However, to date, most microfluidic devices are either still in the development phase, not applicable to all clinical tests, or are not commercially available. To overcome these discrepancies, we describe an all-in-one device for the isolation and multiplexing of clinically applicable CTC assays. Microfilters present an ideal lab-on-a-chip platform for analysis of CTCs as non-toxic and inert materials allow for a multitude of tests from cell growth through clinical staining techniques, all without background interference. Lithographically fabricated microfilters, can be made with high porosity, precise pore dimensions, arrayed pore distribution, and optimized for CTC size-based isolation. In this study we describe microfilter use in isolation and

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