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Tabeshmehr P, Husnain HK, Salmannejad M, et al. Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with H. Transl Neurodegener. 2017;6:29doi: 10.1186/s40035-017-0097-1.
Tabeshmehr, P., Husnain, H. K., Salmannejad, M., Sani, M., Hosseini, S. M., & Khorraminejad Shirazi, M. H. (2017). Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with H. Translational neurodegeneration, 629. https://doi.org/10.1186/s40035-017-0097-1
Tabeshmehr, Parisa, et al. "Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with H." Translational neurodegeneration vol. 6 (2017): 29. doi: https://doi.org/10.1186/s40035-017-0097-1
Tabeshmehr P, Husnain HK, Salmannejad M, Sani M, Hosseini SM, Khorraminejad Shirazi MH. Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with H. Transl Neurodegener. 2017 Oct 30;6:29. doi: 10.1186/s40035-017-0097-1. eCollection 2017. PMID: 29093814; PMCID: PMC5662071.
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Transl Neurodegener. 2017 Oct 30;6:29. doi: 10.1186/s40035-017-0097-1. eCollection 2017.

Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with H.

Translational neurodegeneration

Parisa Tabeshmehr, Haider Kh Husnain, Mahin Salmannejad, Mahsa Sani, Seyed Mojtaba Hosseini, Mohammad Hossein Khorraminejad Shirazi

Affiliations

  1. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
  2. Cell & Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
  3. Department of Basic Sciences, SRU, Riyadh, Saudi Arabia.
  4. Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

PMID: 29093814 PMCID: PMC5662071 DOI: 10.1186/s40035-017-0097-1

Abstract

BACKGROUND: Extensive loss of donor neural stem cell (NSCs) due to ischemic stress and low rate of differentiation at the site of cell graft are two of the major issues that hamper optimal outcome in NSCs transplantation studies. Given that histone deacetylases (HDACs) modulate various cellular processes by deacetylating histones and non-histone proteins, we hypothesized that combined treatment with small molecules, sodium butyrate (NaB; a known HDAC inhibitor) and nicorandil, will enhance the rate neuronal differentiation of NSCs besides their preconditioning to resist oxidative stress.

METHODS: NSCs derived from 14-day old Sprague Dawley rat ganglion eminence were characterized for tri-lineage differentiation. Treatment with 1 mM NaB significantly changed their culture characteristics while continuous treatment for 10 days enhanced their neural differentiation. NaB treatment also preconditioned the cells for their resistance to oxidative stress.

RESULTS: The highest rate of neural differentiation and preconditioning effect was achieved when the NSCs were treated concomitantly with NaB and nicorandil. Cell proliferation assay showed that concomitant treatment with NaB and nicorandil retarded their rate of proliferation.

CONCLUSION: These data conclude that preconditioning of NSCs with NaB and nicorandil effectively enhances their differentiation capacity besides preconditioning the cells to support their survival under ischemic conditions.

Keywords: Apoptosis; Neural stem cells; Oxidative stress; Preconditioning

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