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Kira T, Akahane M, Omokawa S, et al. Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep. World J Orthop. 2017;8(10):754-760doi: 10.5312/wjo.v8.i10.754.
Kira, T., Akahane, M., Omokawa, S., Shimizu, T., Kawate, K., Onishi, T., & Tanaka, Y. (2017). Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep. World journal of orthopedics, 8(10), 754-760. https://doi.org/10.5312/wjo.v8.i10.754
Kira, Tsutomu, et al. "Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep." World journal of orthopedics vol. 8,10 (2017): 754-760. doi: https://doi.org/10.5312/wjo.v8.i10.754
Kira T, Akahane M, Omokawa S, Shimizu T, Kawate K, Onishi T, Tanaka Y. Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep. World J Orthop. 2017 Oct 18;8(10):754-760. doi: 10.5312/wjo.v8.i10.754. eCollection 2017 Oct 18. PMID: 29094005; PMCID: PMC5656490.
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World J Orthop. 2017 Oct 18;8(10):754-760. doi: 10.5312/wjo.v8.i10.754. eCollection 2017 Oct 18.

Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep.

World journal of orthopedics

Tsutomu Kira, Manabu Akahane, Shohei Omokawa, Takamasa Shimizu, Kenji Kawate, Tadanobu Onishi, Yasuhito Tanaka

Affiliations

  1. Department of Orthopedic Surgery, Nara Medical University, Nara 634-8522, Japan. [email protected].
  2. Department of Public Health, Health Management and Policy, Nara Medical University School of Medicine, Nara 634-8522, Japan.
  3. Department of Hand Surgery, Nara Medical University, Nara 634-8522, Japan.
  4. Department of Orthopedic Surgery, Nara Medical University, Nara 634-8522, Japan.
  5. Department of Artificial Joint and Regenerative Medicine for Bone and Cartilage, Nara Medical University, Nara 634-8522, Japan.

PMID: 29094005 PMCID: PMC5656490 DOI: 10.5312/wjo.v8.i10.754

Abstract

AIM: To determine the effects of a cell sheet created from sheep bone marrow and tricalcium phosphate (TCP) on osteogenesis.

METHODS: Bone marrow cells were harvested from a sheep and cultured in a minimal essential medium (MEM) containing ascorbic acid phosphate (AscP) and dexamethasone (Dex). After 2 wk, the formed osteogenic matrix cell sheet was lifted from the culture dish using a scraper. Additionally, harvested bone marrow cells were cultured in MEM only as a negative control group, and in MEM with AscP, Dex, and β-glycerophosphate as a positive control group. For

RESULTS: In the

CONCLUSION: This technique for preparing highly osteoinductive TCP may promote regeneration in large bone defects.

Keywords: Bone marrow; Cell sheet; Mesenchymal stromal cell; Osteogenesis; Sheep

Conflict of interest statement

Conflict-of-interest statement: There is no conflict of interest related to this study.

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