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Gerber PF, Dawson L, Strugnell B, et al. Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs. Vet Med Sci. 2016;3(1):3-12doi: 10.1002/vms3.51.
Gerber, P. F., Dawson, L., Strugnell, B., Burgess, R., Brown, H., & Opriessnig, T. (2017). Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs. Veterinary medicine and science, 3(1), 3-12. https://doi.org/10.1002/vms3.51
Gerber, Priscilla F, et al. "Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs." Veterinary medicine and science vol. 3,1 (2017): 3-12. doi: https://doi.org/10.1002/vms3.51
Gerber PF, Dawson L, Strugnell B, Burgess R, Brown H, Opriessnig T. Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs. Vet Med Sci. 2016 Dec 16;3(1):3-12. doi: 10.1002/vms3.51. eCollection 2017 Feb. PMID: 29067204; PMCID: PMC5645835.
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Vet Med Sci. 2016 Dec 16;3(1):3-12. doi: 10.1002/vms3.51. eCollection 2017 Feb.

Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs.

Veterinary medicine and science

Priscilla F Gerber, Lorna Dawson, Ben Strugnell, Robert Burgess, Helen Brown, Tanja Opriessnig

Affiliations

  1. The Roslin Institute and The Royal (Dick) School of Veterinary StudiesUniversity of EdinburghMidlothianScotlandUK.
  2. School of AgricultureFood and Rural DevelopmentNewcastle UniversityNewcastle upon TyneUK.
  3. Evidence-based Veterinary Consultancy (EBVC) Ltd.Rural Enterprise CentreRedhillsPenrithCumbriaUK.
  4. Department of Veterinary Diagnostic and Production Animal MedicineIowa State UniversityAmesIowaUSA.

PMID: 29067204 PMCID: PMC5645835 DOI: 10.1002/vms3.51

Abstract

Influenza A virus (IAV) is economically important in pig production and has broad public health implications. In Europe, active IAV surveillance includes demonstration of antigen in nasal swabs and/or demonstration of antibodies in serum (SER) samples; however, collecting appropriate numbers of individual pig samples can be costly and labour-intensive. The objective of this study was to compare the probability of detecting IAV antibody positive populations using SER versus oral fluid (OF) samples. Paired pen samples, one OF and 5-14 SER samples, were collected cross-sectional or longitudinally. A commercial nucleoprotein (NP)-based blocking ELISA was used to test 244 OF and 1004 SER samples from 123 pens each containing 20-540 pigs located in 27 UK herds. Overall, the IAV antibody detection rate was higher in SER samples compared to OFs under the study conditions. Pig age had a significant effect on the probability of detecting positive pens. For 3-9-week-old pigs the probability of detecting IAV antibody positive samples in a pen with 95% confidence intervals was 40% (23-60) for OF and 61% (0.37-0.80) for SER (

Keywords: Swine influenza virus; diagnostics; enzyme‐linked immunosorbent assay; surveillance

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