Display options
Cite
Sakata H, Okamoto A, Aoyama-Ishikawa M, et al. Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction. Acute Med Surg. 2016;4(1):38-45doi: 10.1002/ams2.218.
Sakata, H., Okamoto, A., Aoyama-Ishikawa, M., Yamashita, H., Kohama, K., Fujisaki, N., Yamada, T., Kotani, J., Tsukahara, K., Iida, A., & Nakao, A. (2017). Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction. Acute medicine & surgery, 4(1), 38-45. https://doi.org/10.1002/ams2.218
Sakata, Hiroyuki, et al. "Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction." Acute medicine & surgery vol. 4,1 (2017): 38-45. doi: https://doi.org/10.1002/ams2.218
Sakata H, Okamoto A, Aoyama-Ishikawa M, Yamashita H, Kohama K, Fujisaki N, Yamada T, Kotani J, Tsukahara K, Iida A, Nakao A. Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction. Acute Med Surg. 2016 Jun 14;4(1):38-45. doi: 10.1002/ams2.218. eCollection 2017 Jan. PMID: 29123834; PMCID: PMC5667287.
Copy Download .nbib Format: NLM
Share it on

Acute Med Surg. 2016 Jun 14;4(1):38-45. doi: 10.1002/ams2.218. eCollection 2017 Jan.

Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction.

Acute medicine & surgery

Hiroyuki Sakata, Ayana Okamoto, Michiko Aoyama-Ishikawa, Hayato Yamashita, Keisuke Kohama, Noritomo Fujisaki, Taihei Yamada, Joji Kotani, Kohei Tsukahara, Atsuyoshi Iida, Atsunori Nakao

Affiliations

  1. Department of Emergency Disaster and Critical Care Medicine Hyogo College of Medicine Nishinomiya Hyogo Japan.
  2. Kobe University Graduate School of Health Science Kobe Hyogo Japan.
  3. Senri Critical Care Medical Center Saiseikai Senri Hospital Suita Osaka Japan.
  4. Department of Emergency and Critical Care Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama, Okayama Japan.

PMID: 29123834 PMCID: PMC5667287 DOI: 10.1002/ams2.218

Abstract

AIM: Gastrointestinal dysmotility frequently occurs during sepsis and multiple organ failure, remaining a major cause of morbidity and mortality in critically ill patients. Previous studies have shown that hydrogen, a new therapeutic gas, can improve various organ damage associated with sepsis. In this study, we investigated the protective efficacies of inhaled hydrogen against lipopolysaccharide (LPS)-induced ileus.

METHODS: Sepsis was induced in rats and mice by a single i.p. injection of LPS at 15 mg/kg for mice and 5 mg/kg for rats. Four groups of rats and mice including sham/air, sham/hydrogen, LPS/air, and LPS/hydrogen were analyzed. Hydrogen (1.3%) was inhaled for 25 h beginning at 1 h prior to LPS treatment. Gastrointestinal transit was quantified and cytokine levels, as well as neutrophil extravasation, in the intestinal muscularis propria were determined.

RESULTS: Lipopolysaccharide challenge remarkably delayed gastrointestinal transit of non-absorbable dextran, associated with increased leukocyte recruitment and upregulation of pro-inflammatory cytokine mRNA expressions in the muscularis propria. Hydrogen significantly prevented LPS-induced bowel dysmotility and reduced leukocyte extravasation, as well as inhibition of inflammatory cytokine expression.

CONCLUSIONS: This study showed the protective effects of hydrogen inhalation on LPS-induced septic ileus through inhibition of inflammation in the muscularis propria. These inhibitory effects on the pro-inflammatory response may be partially derived from anti-inflammatory cytokine interleukin-10 induction.

Keywords: Hydrogen; ileus; inflammation; interleukin‐10; sepsis

References

  1. J Clin Invest. 1997 Apr 1;99(7):1516-24 - PubMed
  2. Am J Physiol Lung Cell Mol Physiol. 2013 May 15;304(10 ):L646-56 - PubMed
  3. Crit Care. 2010;14(6):R234 - PubMed
  4. Am J Physiol. 1999 Aug;277(2 Pt 1):G478-86 - PubMed
  5. Biochem Biophys Res Commun. 2010 Jul 2;397(3):608-13 - PubMed
  6. J Exp Med. 1998 Mar 16;187(6):917-28 - PubMed
  7. Neurogastroenterol Motil. 2010 Mar;22(3):232-45 - PubMed
  8. Gastroenterology. 2003 Feb;124(2):377-91 - PubMed
  9. Curr Gastroenterol Rep. 2011 Aug;13(4):358-67 - PubMed
  10. Gastroenterology. 2004 Aug;127(2):595-606 - PubMed
  11. Gastroenterology. 2000 Feb;118(2):316-27 - PubMed
  12. Am J Pathol. 2003 Oct;163(4):1587-98 - PubMed
  13. J Pharmacol Exp Ther. 2006 Dec;319(3):1265-75 - PubMed
  14. Surgery. 2015 Aug;158(2):399-407 - PubMed
  15. Am J Physiol Gastrointest Liver Physiol. 2002 Jan;282(1):G145-55 - PubMed
  16. Surgery. 1990 Apr;107(4):397-410 - PubMed
  17. J Dig Dis. 2014 Jul;15(7):335-44 - PubMed
  18. Crit Care Med. 2013 Feb;41(2):580-637 - PubMed

Publication Types