Anal Chem. 2017 Dec 19;89(24):13320-13325. doi: 10.1021/acs.analchem.7b03484. Epub 2017 Dec 05.

Coupling Microchip Electrospray Ionization Devices with High Pressure Mass Spectrometry.

Analytical chemistry

William M Gilliland, J Scott Mellors, J Michael Ramsey

PMID: 29151340 DOI: 10.1021/acs.analchem.7b03484

Abstract

A microchip electrospray ionization source was coupled with high pressure mass spectrometry (HPMS). A continuous atmospheric inlet consisting of a stainless steel capillary and DC ion optics was designed to conduct ions into the mass spectrometer. Infusions of amino acids and peptides were performed and detected with a miniature cylindrical ion trap (mini-CIT)-based mass spectrometer operated at ≥1 Torr with air as the buffer gas. Detection of glycine and thymopentin (separately) demonstrated the mass range of the mini-CIT detector could span from m/z 75 to 681. A microchip capillary electrophoresis (CE) separation with mini-CIT detection was performed, and the results were compared with detection using a commercial instrument (Waters Synapt G2). Comparable separation efficiencies were observed with both mass spectrometers as detectors, with about 6 times better signal-to-noise observed on the Synapt G2. Comparison of mass spectra in the two systems reveals similar features observed, but with wider peak widths in the mini-CIT than on the Synapt G2 as expected due to high-pressure operation.

Publication Types

• Journal Article
• Research Support, U.S. Gov't, Non-P.H.S.