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Mahbub MM, Chowdhury SM, Christensen SM. Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8. Mob DNA. 2017;8:16doi: 10.1186/s13100-017-0097-9.
Mahbub, M. M., Chowdhury, S. M., & Christensen, S. M. (2017). Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8. Mobile DNA, 816. https://doi.org/10.1186/s13100-017-0097-9
Mahbub, M Murshida, et al. "Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8." Mobile DNA vol. 8 (2017): 16. doi: https://doi.org/10.1186/s13100-017-0097-9
Mahbub MM, Chowdhury SM, Christensen SM. Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8. Mob DNA. 2017 Nov 07;8:16. doi: 10.1186/s13100-017-0097-9. eCollection 2017. PMID: 29151899; PMCID: PMC5678591.
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Mob DNA. 2017 Nov 07;8:16. doi: 10.1186/s13100-017-0097-9. eCollection 2017.

Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8.

Mobile DNA

M Murshida Mahbub, Saiful M Chowdhury, Shawn M Christensen

Affiliations

  1. Department of Biology, University of Texas at Arlington, 501 S. Nedderman Drive, Room 337, Arlington, TX 76010 USA.
  2. Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Room 130, Arlington, TX 76010 USA.

PMID: 29151899 PMCID: PMC5678591 DOI: 10.1186/s13100-017-0097-9

Abstract

BACKGROUND: R2 elements are a clade of early branching Long Interspersed Elements (LINEs). LINEs are retrotransposable elements whose replication can have profound effects on the genomes in which they reside. No crystal or EM structures exist for the reverse transcriptase (RT) and linker regions of LINEs.

RESULTS: Using limited proteolysis as a probe for globular domain structure, we show that the protein encoded by the

CONCLUSIONS: The protein encoded by RLE LINEs consists of two major globular domains. The larger of the two globular domain contains the RT, linker, and RLE and is similar to the large fragment of the spliceosomal protein Prp8. The similarities are suggestive of possible common ancestry.

Keywords: Line; Non-LTR retrotransposon; RNA splicing; Reverse transcriptase; Target primed reverse transcription (TPRT); Transposable element (TE)

References

  1. RNA. 2011 May;17(5):799-808 - PubMed
  2. Cell. 1996 Nov 29;87(5):905-16 - PubMed
  3. Curr Biol. 2006 Jul 11;16(13):1280-9 - PubMed
  4. Nat Protoc. 2006;1(6):2856-60 - PubMed
  5. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20311-6 - PubMed
  6. Nucleic Acids Res. 2016 Apr 20;44(7):3276-87 - PubMed
  7. Nat Protoc. 2015 Jun;10(6):845-58 - PubMed
  8. Mob DNA. 2012 Nov 21;3(1):19 - PubMed
  9. Nature. 2008 Oct 2;455(7213):633-7 - PubMed
  10. RNA. 2010 Apr;16(4):732-47 - PubMed
  11. Nature. 2016 Sep 8;537(7619):197-201 - PubMed
  12. Nature. 2017 Feb 16;542(7641):318-323 - PubMed
  13. Nat Struct Mol Biol. 2014 Jun;21(6):507-12 - PubMed
  14. RNA. 2005 Jan;11(1):14-28 - PubMed
  15. Anal Chem. 1996 Mar 1;68(5):850-8 - PubMed
  16. Science. 2016 Jan 29;351(6272):466-75 - PubMed
  17. Nucleic Acids Res. 2007;35(14):4914-26 - PubMed
  18. RNA. 2004 Sep;10(9):1433-43 - PubMed
  19. Mob Genet Elements. 2011 Sep;1(3):169-178 - PubMed
  20. RNA. 2013 Jul;19(7):958-70 - PubMed
  21. Virus Res. 2017 Apr 15;234:34-43 - PubMed
  22. J Comput Chem. 2004 Oct;25(13):1605-12 - PubMed
  23. Nucleic Acids Res. 2016 Jul 8;44(W1):W410-5 - PubMed
  24. Nature. 2013 Jan 31;493(7434):638-43 - PubMed
  25. Structure. 2004 Jun;12 (6):975-86 - PubMed
  26. Curr Opin Struct Biol. 2014 Apr;25:57-66 - PubMed
  27. Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17602-7 - PubMed
  28. Int J Mol Sci. 2015 Jun 08;16(6):12943-57 - PubMed
  29. Microbiol Spectr. 2015 Apr;3(2):MDNA3-0058-2014 - PubMed
  30. Nucleic Acids Res. 2013 Dec;41(22):10563-72 - PubMed
  31. Hum Mutat. 2007 Jun;28(6):527-39 - PubMed
  32. J Biol Chem. 1999 Jan 29;274(5):2706-16 - PubMed
  33. EMBO J. 1990 Oct;9(10):3353-62 - PubMed
  34. J Biol Chem. 2008 Aug 29;283(35):24089-102 - PubMed
  35. J Biol Chem. 2007 Aug 24;282(34):24893-904 - PubMed
  36. Nucleic Acids Res. 2010 Sep;38(17):5609-22 - PubMed
  37. Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):731-6 - PubMed
  38. Cell. 1996 Nov 29;87(5):917-27 - PubMed
  39. Microbiol Spectr. 2015 Apr;3(2):MDNA3-0061-2014 - PubMed
  40. Anal Chem. 2003 Mar 15;75(6):1300-6 - PubMed
  41. Anal Biochem. 1978 Jun 1;86(2):655-64 - PubMed
  42. Microbiol Spectr. 2015 Feb;3(1):MDNA3-0050-2014 - PubMed
  43. FEBS Open Bio. 2013 Sep 21;3:433-7 - PubMed
  44. Cytogenet Genome Res. 2005;110(1-4):250-68 - PubMed
  45. Nat Methods. 2012 Jun 28;9(7):676-82 - PubMed
  46. Bioinformatics. 2014 Nov 15;30(22):3276-8 - PubMed
  47. Nat Struct Mol Biol. 2016 Jun;23 (6):549-57 - PubMed
  48. Nature. 2016 Feb 18;530(7590):298-302 - PubMed
  49. Mol Biol Evol. 1999 Apr;16(4):502-11 - PubMed
  50. Microbiol Spectr. 2015 Apr;3(2):MDNA3-0011-2014 - PubMed
  51. Rapid Commun Mass Spectrom. 2014 Mar 30;28(6):635-44 - PubMed
  52. Mob DNA. 2015 Jun 02;6:11 - PubMed
  53. J Mol Biol. 1997 Aug 22;271(3):311-32 - PubMed
  54. J Biol Chem. 2002 Jan 11;277(2):1381-7 - PubMed
  55. Nucleic Acids Res. 2003 Jan 15;31(2):532-50 - PubMed
  56. Nat Struct Mol Biol. 2011 Aug 07;18(9):1006-14 - PubMed
  57. Cell. 1993 Feb 26;72(4):595-605 - PubMed
  58. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):E4005-14 - PubMed
  59. PLoS Genet. 2010 Oct 07;6(10):null - PubMed
  60. Nat Struct Mol Biol. 2010 Apr;17(4):513-8 - PubMed
  61. Nucleic Acids Res. 2014 Jul;42(13):8405-15 - PubMed
  62. PLoS Pathog. 2013;9(8):e1003549 - PubMed
  63. Methods Mol Biol. 2014;1079:263-71 - PubMed
  64. EMBO J. 2004 Sep 1;23(17):3462-71 - PubMed
  65. Mol Cell Biol. 2005 Aug;25(15):6617-28 - PubMed
  66. Bioinformatics. 2007 Nov 1;23(21):2947-8 - PubMed
  67. Nat Struct Mol Biol. 2016 Jun;23 (6):558-65 - PubMed
  68. PLoS One. 2011;6(5):e19541 - PubMed
  69. Microbiol Spectr. 2015 Apr;3(2):MDNA3-0001-2014 - PubMed

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