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Kempf O, Kempf K, Schobert R, et al. Hydrodabcyl: A Superior Hydrophilic Alternative to the Dark Fluorescence Quencher Dabcyl. Anal Chem. 2017;89(22):11893-11897doi: 10.1021/acs.analchem.7b03488.
Kempf, O., Kempf, K., Schobert, R., & Bombarda, E. (2017). Hydrodabcyl: A Superior Hydrophilic Alternative to the Dark Fluorescence Quencher Dabcyl. Analytical chemistry, 89(22), 11893-11897. https://doi.org/10.1021/acs.analchem.7b03488
Kempf, Oxana, et al. "Hydrodabcyl: A Superior Hydrophilic Alternative to the Dark Fluorescence Quencher Dabcyl." Analytical chemistry vol. 89,22 (2017): 11893-11897. doi: https://doi.org/10.1021/acs.analchem.7b03488
Kempf O, Kempf K, Schobert R, Bombarda E. Hydrodabcyl: A Superior Hydrophilic Alternative to the Dark Fluorescence Quencher Dabcyl. Anal Chem. 2017 Nov 21;89(22):11893-11897. doi: 10.1021/acs.analchem.7b03488. Epub 2017 Nov 07. PMID: 29069895.
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Anal Chem. 2017 Nov 21;89(22):11893-11897. doi: 10.1021/acs.analchem.7b03488. Epub 2017 Nov 07.

Hydrodabcyl: A Superior Hydrophilic Alternative to the Dark Fluorescence Quencher Dabcyl.

Analytical chemistry

Oxana Kempf, Karl Kempf, Rainer Schobert, Elisa Bombarda

Affiliations

  1. Department of Biochemistry and ‡Organic Chemistry Laboratory, University of Bayreuth , Universitaetsstrasse 30, 95440, Bayreuth, Germany.

PMID: 29069895 DOI: 10.1021/acs.analchem.7b03488

Abstract

Dark fluorescence quenchers are nonfluorescent dyes that can modulate the fluorescence signal of an appropriate fluorophore donor in a distance-dependent manner. Dark quenchers are extensively used in many biomolecular analytical applications, such as studies with fluorogenic protease substrates or nucleic acids probes. A very popular dark fluorescence quencher is dabcyl, which is a hydrophobic azobenzene derivative. However, its insolubility in water may constitute a major drawback, especially during the investigation of biochemical systems whose natural solvent is water. We designed and synthesized a new azobenzene-based dark quencher with excellent solubility in aqueous media, which represents a superior alternative to the much-used dabcyl. The advantage of hydrodabcyl over dabcyl is exemplarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the proteases thermolysin and papain.

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