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Gatto F, Cagliani R, Catelani T, et al. PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles. Nanomaterials (Basel). 2017;7(10)doi: 10.3390/nano7100332.
Gatto, F., Cagliani, R., Catelani, T., Guarnieri, D., Moglianetti, M., Pompa, P. P., & Bardi, G. (2017). PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles. Nanomaterials (Basel, Switzerland), 7(10), . https://doi.org/10.3390/nano7100332
Gatto, Francesca, et al. "PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles." Nanomaterials (Basel, Switzerland) vol. 7,10 (2017). doi: https://doi.org/10.3390/nano7100332
Gatto F, Cagliani R, Catelani T, Guarnieri D, Moglianetti M, Pompa PP, Bardi G. PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles. Nanomaterials (Basel). 2017 Oct 17;7(10). doi: 10.3390/nano7100332. PMID: 29039753; PMCID: PMC5666497.
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Nanomaterials (Basel). 2017 Oct 17;7(10). doi: 10.3390/nano7100332.

PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles.

Nanomaterials (Basel, Switzerland)

Francesca Gatto, Roberta Cagliani, Tiziano Catelani, Daniela Guarnieri, Mauro Moglianetti, Pier Paolo Pompa, Giuseppe Bardi

Affiliations

  1. Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].
  2. Department of Engineering for Innovation, University of Salento, Via per Monteroni, 73010 Lecce, Italy. [email protected].
  3. Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].
  4. Department of Chemistry and Industrial Chemistry, University of Genova, Via Dodecaneso 31, 16146 Genova, Italy. [email protected].
  5. Electron Microscopy Laboratory, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].
  6. Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].
  7. Nanobiointeractions & Nanodiagnostics, Center for Bio-Molecular Nanotechnologies, Istituto Italiano di Tecnologia, Via Barsanti, 73010 Arnesano, Lecce, Italy. [email protected].
  8. Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].
  9. Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. [email protected].

PMID: 29039753 PMCID: PMC5666497 DOI: 10.3390/nano7100332

Abstract

The innate immune system consists of several complex cellular and molecular mechanisms. During inflammatory responses, blood-circulating monocytes are driven to the sites of inflammation, where they differentiate into tissue macrophages. The research of novel nanomaterials applied to biomedical sciences is often limited by their toxicity or dangerous interactions with the immune cell functions. Platinum nanoparticles (PtNPs) have shown efficient antioxidant properties within several cells, but information on their potential harmful role in the monocyte-to-macrophage differentiation process is still unknown. Here, we studied the morphology and the release of cytokines in PMA-differentiated THP-1 pre-treated with 5 nm PtNPs. Although NP endocytosis was evident, we did not find differences in the cellular structure or in the release of inflammatory cytokines and chemokines compared to cells differentiated in PtNP-free medium. However, the administration of PtNPs to previously differentiated THP-1 induced massive phagocytosis of the PtNPs and a slight metabolism decrease at higher doses. Further investigation using undifferentiated and differentiated neutrophil-like HL60 confirmed the harmlessness of PtNPs with non-adherent innate immune cells. Our results demonstrate that citrate-coated PtNPs are not toxic with these immune cell lines, and do not affect the PMA-stimulated THP-1 macrophage differentiation process in vitro.

Keywords: chemokines; cytokines; differentiation; macrophages; platinum nanoparticles

Conflict of interest statement

The authors declare no conflict of interest.

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