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Alvarez-Zúñiga MT, Santiago-Hernández A, Rodríguez-Mendoza J, et al. Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA. AMB Express. 2017;7(1):194doi: 10.1186/s13568-017-0494-y.
Alvarez-Zúñiga, M. T., Santiago-Hernández, A., Rodríguez-Mendoza, J., Campos, J. E., Pavón-Orozco, P., Trejo-Estrada, S., & Hidalgo-Lara, M. E. (2017). Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA. AMB Express, 7(1), 194. https://doi.org/10.1186/s13568-017-0494-y
Alvarez-Zúñiga, María Teresa, et al. "Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA." AMB Express vol. 7,1 (2017): 194. doi: https://doi.org/10.1186/s13568-017-0494-y
Alvarez-Zúñiga MT, Santiago-Hernández A, Rodríguez-Mendoza J, Campos JE, Pavón-Orozco P, Trejo-Estrada S, Hidalgo-Lara ME. Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA. AMB Express. 2017 Nov 02;7(1):194. doi: 10.1186/s13568-017-0494-y. PMID: 29098440; PMCID: PMC5668220.
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AMB Express. 2017 Nov 02;7(1):194. doi: 10.1186/s13568-017-0494-y.

Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA.

AMB Express

María Teresa Alvarez-Zúñiga, Alejandro Santiago-Hernández, Johan Rodríguez-Mendoza, Jorge E Campos, Patricia Pavón-Orozco, Sergio Trejo-Estrada, María Eugenia Hidalgo-Lara

Affiliations

  1. Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Av. Instituto Politécnico Nacional No. 2508, 07360, Mexico City, Mexico.
  2. Laboratorio de Bioquímica Molecular UBIPRO., FES Iztacala, UNAMAv., de los Barrios No. 1, Los Reyes Iztacala, 54090, Tlanepantla, Estado de Mexico, Mexico.
  3. Facultad de Ciencias Químicas, Universidad Veracruzana, Av. Universidad Km, 7.5., 96538, Coatzacoalcos, Veracruz, Mexico.
  4. Centro de Investigación en Biotecnología Aplicada-IPN, Km 1.5 Carretera Estatal Tecuexcomac-Tepetitla, 90700, Tepetitla, Tlaxcala, Mexico.
  5. Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Av. Instituto Politécnico Nacional No. 2508, 07360, Mexico City, Mexico. [email protected].

PMID: 29098440 PMCID: PMC5668220 DOI: 10.1186/s13568-017-0494-y

Abstract

The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ITS2. L. ramosa H71D was able to grow over a wide range of temperatures (25-45 °C), manifesting optimal growth at 37 °C. A 64 kDa xylanase (named LrXynA) was purified from the culture supernatant of L. ramosa H71D grown on 2% carboxymethylcellulose (CMC), as the only carbon source. LrXynA displayed optimal activity at pH 6 and temperature of 65 °C. The enzyme retained more than 50% of its maximal activity over a broad range of pH values (4.5-7.5). Enzyme half-life (t

Keywords: Internal transcribed spacer (ITS); Lichtheimia ramosa; Sugarcane hydrolysis; Xylanase; Zygomycete fungus

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