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Østevold K, Meléndez AV, Lehmann F, et al. Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells. Oncotarget. 2017;8(44):76686-76698doi: 10.18632/oncotarget.20805.
Østevold, K., Meléndez, A. V., Lehmann, F., Schmidt, G., Aktories, K., & Schwan, C. (2017). Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells. Oncotarget, 8(44), 76686-76698. https://doi.org/10.18632/oncotarget.20805
Østevold, Kristine, et al. "Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells." Oncotarget vol. 8,44 (2017): 76686-76698. doi: https://doi.org/10.18632/oncotarget.20805
Østevold K, Meléndez AV, Lehmann F, Schmidt G, Aktories K, Schwan C. Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells. Oncotarget. 2017 Sep 11;8(44):76686-76698. doi: 10.18632/oncotarget.20805. eCollection 2017 Sep 29. PMID: 29100341; PMCID: PMC5652735.
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Oncotarget. 2017 Sep 11;8(44):76686-76698. doi: 10.18632/oncotarget.20805. eCollection 2017 Sep 29.

Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells.

Oncotarget

Kristine Østevold, Ana V Meléndez, Friederike Lehmann, Gudula Schmidt, Klaus Aktories, Carsten Schwan

Affiliations

  1. Institute of Experimental and Clinical Pharmacology and Toxicology, Medical Faculty, University of Freiburg, 79104 Freiburg, Germany.
  2. Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
  3. Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 79104 Freiburg, Germany.
  4. Faculty of Chemistry and Pharmacy, University of Freiburg, 79104 Freiburg, Germany.
  5. Centre for Biological Signalling Studies (BIOSS), University of Freiburg, 79104 Freiburg, Germany.

PMID: 29100341 PMCID: PMC5652735 DOI: 10.18632/oncotarget.20805

Abstract

Microtentacles are mostly microtubule-based cell protrusions that are formed by detached tumor cells. Here, we report that the formation of tumor cell microtentacles depends on the presence and dynamics of guanine nucleotide-binding proteins of the septin family, which are part of the cytoskeleton. In matrix-attached breast, lung, prostate and pancreas cancer cells, septins are associated with the cytosolic actin cytoskeleton. Detachment of cells causes redistribution of septins to the membrane, where microtentacle formation occurs. Forchlorfenuron, which inhibits septin functions, blocks microtentacle formation. The small GTPase Cdc42 and its effector proteins Borgs regulate septins and are essential for microtentacle formation. Dominant active and inactive Cdc42 inhibit microtentacle formation indicating that the free cycling of Cdc42 between its active and inactive state is essential for septin regulation and microtentacle formation. Cell attachment and aggregation models suggest that septins play an essential role in the metastatic behavior of tumor cells.

Keywords: Clostridium difficile toxin; actin ADP-ribosylation; microtentacles; microtubules; septin

Conflict of interest statement

CONFLICTS OF INTEREST None.

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