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Carvalho NS, Moron AF, Menon R, et al. Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. Exp Ther Med. 2017;14(4):3732-3736doi: 10.3892/etm.2017.4976.
Carvalho, N. S., Moron, A. F., Menon, R., Cavalheiro, S., Barbosa, M. M., Milani, H. J., & Ishigai, M. M. (2017). Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. Experimental and therapeutic medicine, 14(4), 3732-3736. https://doi.org/10.3892/etm.2017.4976
Carvalho, Natalia S, et al. "Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele." Experimental and therapeutic medicine vol. 14,4 (2017): 3732-3736. doi: https://doi.org/10.3892/etm.2017.4976
Carvalho NS, Moron AF, Menon R, Cavalheiro S, Barbosa MM, Milani HJ, Ishigai MM. Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. Exp Ther Med. 2017 Oct;14(4):3732-3736. doi: 10.3892/etm.2017.4976. Epub 2017 Aug 21. PMID: 29042971; PMCID: PMC5639275.
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Exp Ther Med. 2017 Oct;14(4):3732-3736. doi: 10.3892/etm.2017.4976. Epub 2017 Aug 21.

Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele.

Experimental and therapeutic medicine

Natalia S Carvalho, Antonio F Moron, Ramkumar Menon, Sergio Cavalheiro, Mauricio M Barbosa, Herbene J Milani, Marcia M Ishigai

Affiliations

  1. Department of Obstetrics, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, SP 04021-001, Brazil.
  2. Fetal Medicine Division, Hospital e Maternidade Santa Joana, São Paulo, SP 04103-000, Brazil.
  3. Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX 77555, USA.
  4. Department of Neurosurgery, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, SP 04021-001, Brazil.
  5. Department of Pathology, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, SP 04021-001, Brazil.

PMID: 29042971 PMCID: PMC5639275 DOI: 10.3892/etm.2017.4976

Abstract

An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non-surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.

Keywords: fetal membrane healing; fetal membrane repair; myelomeningocele; open fetal surgery; spina bifida

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