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Ramakrishnan GS, Fathima AA, Ramya M. A rapid and efficient DNA extraction method suitable for marine macroalgae. 3 Biotech. 2017;7(6):364doi: 10.1007/s13205-017-0992-2.
Ramakrishnan, G. S., Fathima, A. A., & Ramya, M. (2017). A rapid and efficient DNA extraction method suitable for marine macroalgae. 3 Biotech, 7(6), 364. https://doi.org/10.1007/s13205-017-0992-2
Ramakrishnan, Gautham Subramaniam, et al. "A rapid and efficient DNA extraction method suitable for marine macroalgae." 3 Biotech vol. 7,6 (2017): 364. doi: https://doi.org/10.1007/s13205-017-0992-2
Ramakrishnan GS, Fathima AA, Ramya M. A rapid and efficient DNA extraction method suitable for marine macroalgae. 3 Biotech. 2017 Dec;7(6):364. doi: 10.1007/s13205-017-0992-2. Epub 2017 Oct 04. PMID: 29043116; PMCID: PMC5628057.
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3 Biotech. 2017 Dec;7(6):364. doi: 10.1007/s13205-017-0992-2. Epub 2017 Oct 04.

A rapid and efficient DNA extraction method suitable for marine macroalgae.

3 Biotech

Gautham Subramaniam Ramakrishnan, Anwar Aliya Fathima, Mohandass Ramya

Affiliations

  1. Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu 603203 India.

PMID: 29043116 PMCID: PMC5628057 DOI: 10.1007/s13205-017-0992-2

Abstract

Macroalgae are a diverse group of organisms. Marine macroalgae, in particular, have numerous medicinal and industrial applications. Molecular studies of macroalgae require suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from macroalgae. However, they are either time consuming, expensive or work only with few species. The method described in this study is rapid and efficient and applicable to different types of marine macroalgae. This method yields an average of 3.85 µg of DNA per 50 mg of algal tissue, with an average purity of 1.88. The isolated DNA was suitable for PCR amplification of universal plastid region of macroalgae.

Keywords: Chlorophyta; DNA extraction; Genomic DNA; Macroalgae; Phaeophyceae; Rhodophyta

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