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Chem Sci. 2015 Aug 01;6(8):4681-4689. doi: 10.1039/c5sc01124h. Epub 2015 May 26.

Systematic and site-specific analysis of .

Chemical science

Weixuan Chen, Johanna M Smeekens, Ronghu Wu

Affiliations

  1. School of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience , Georgia Institute of Technology , Atlanta , Georgia 30332 , USA . Email: [email protected] ; ; Tel: +1-404-385-1515.

PMID: 29142707 PMCID: PMC5667505 DOI: 10.1039/c5sc01124h

Abstract

Glycoproteins on the cell surface are ubiquitous and essential for cells to interact with the extracellular matrix, communicate with other cells, and respond to environmental cues. Although surface sialoglycoproteins can dramatically impact cell properties and represent different cellular statuses, global and site-specific analysis of sialoglycoproteins only on the cell surface is extraordinarily challenging. An effective method integrating metabolic labeling, copper-free click chemistry and mass spectrometry-based proteomics was developed to globally and site-specifically analyze surface

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