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Jiang W, Macmillan H, Madec AM, et al. Purification and characterization of GAD65-specific monoclonal autoantibodies. F1000Res. 2015;4:135doi: 10.12688/f1000research.6467.1.
Jiang, W., Macmillan, H., Madec, A. M., & Mellins, E. D. (2015). Purification and characterization of GAD65-specific monoclonal autoantibodies. F1000Research, 4135. https://doi.org/10.12688/f1000research.6467.1
Jiang, Wei, et al. "Purification and characterization of GAD65-specific monoclonal autoantibodies." F1000Research vol. 4 (2015): 135. doi: https://doi.org/10.12688/f1000research.6467.1
Jiang W, Macmillan H, Madec AM, Mellins ED. Purification and characterization of GAD65-specific monoclonal autoantibodies. F1000Res. 2015 May 29;4:135. doi: 10.12688/f1000research.6467.1. eCollection 2015. PMID: 29167731; PMCID: PMC5680538.
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F1000Res. 2015 May 29;4:135. doi: 10.12688/f1000research.6467.1. eCollection 2015.

Purification and characterization of GAD65-specific monoclonal autoantibodies.

F1000Research

Wei Jiang, Henriette Macmillan, Anne-Marie Madec, Elizabeth D Mellins

Affiliations

  1. Department of Pediatrics, Stanford University, Stanford, CA, 94305, USA.
  2. Stanford Program in Immunology, Stanford University, Stanford, CA, 94305, USA.
  3. Department of Medicine, University of California San Francisco, San Francisco, CA, 94143, USA.
  4. INSERM U1060, Faculté de médecine Lyon-Sud, Oullins Cedex, France.

PMID: 29167731 PMCID: PMC5680538 DOI: 10.12688/f1000research.6467.1

Abstract

Autoantibodies against antigens expressed by insulin-producing β cells are circulating in both healthy individuals and patients at risk of developing Type 1 diabetes. Recent studies suggest that another set of antibodies (anti-idiotypic antibodies) exists in this antibody/antigen interacting network to regulate auto-reactive responses. Anti-idiotypic antibodies may block the antigen-binding site of autoantibodies or inhibit autoantibody expression and secretion. The equilibrium between autoantibodies and anti-idiotypic antibodies plays a critical role in mediating or preventing autoimmunity. Herein, using GAD65/anti-GAD65 autoantibodies as a model system, we aimed at establishing reliable approaches for purification of highly pure autoantibodies for the downstream investigation of molecular mechanisms underlying such a network.

Keywords: GAD65-specific; affinity purification; autoantibody production; monoclonal autoantibody

Conflict of interest statement

Competing interests: No competing interests were disclosed.

References

  1. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2832-6 - PubMed
  2. Clin Exp Immunol. 1999 Dec;118(3):349-56 - PubMed
  3. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5471-6 - PubMed
  4. Autoimmunity. 2008 Feb;41(1):11-8 - PubMed
  5. Diabetes. 2001 Sep;50(9):1976-82 - PubMed
  6. Diabetes. 2003 Nov;52(11):2689-95 - PubMed
  7. Diabetes. 2008 May;57(5):1293-301 - PubMed
  8. J Clin Endocrinol Metab. 1997 Aug;82(8):2664-70 - PubMed
  9. J Mol Biol. 1999 Apr 16;287(5):983-99 - PubMed
  10. PLoS One. 2012;7(2):e32515 - PubMed
  11. Mol Immunol. 2007 Feb;44(6):1178-89 - PubMed
  12. J Immunol. 1996 May 1;156(9):3541-9 - PubMed
  13. PLoS One. 2013 Jun 13;8(6):e65173 - PubMed

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