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Di Giancamillo A, Deponti D, Gervaso F, et al. The analysis of different scaffolds and the benefit of fibrin glue for tendon tissue engineering at different culture times. J Biol Regul Homeost Agents. 2017;31(4):67-73
Di Giancamillo, A., Deponti, D., Gervaso, F., Salvatore, L., Scalera, F., Mangiavini, L., Scurati, R., Sannino, A., & Peretti, G. M. (2017). The analysis of different scaffolds and the benefit of fibrin glue for tendon tissue engineering at different culture times. Journal of biological regulators and homeostatic agents, 31(4), 67-73.
Di Giancamillo, A, et al. "The analysis of different scaffolds and the benefit of fibrin glue for tendon tissue engineering at different culture times." Journal of biological regulators and homeostatic agents vol. 31,4 (2017): 67-73.
Di Giancamillo A, Deponti D, Gervaso F, Salvatore L, Scalera F, Mangiavini L, Scurati R, Sannino A, Peretti GM. The analysis of different scaffolds and the benefit of fibrin glue for tendon tissue engineering at different culture times. J Biol Regul Homeost Agents. 2017 Oct-Dec,;31(4):67-73. PMID: 29185298.
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J Biol Regul Homeost Agents. 2017 Oct-Dec,;31(4):67-73.

The analysis of different scaffolds and the benefit of fibrin glue for tendon tissue engineering at different culture times.

Journal of biological regulators and homeostatic agents

A Di Giancamillo, D Deponti, F Gervaso, L Salvatore, F Scalera, L Mangiavini, R Scurati, A Sannino, G M Peretti

Affiliations

  1. Department of Health, Animal Science and Food Safety, University of Milan, Italy
  2. IRCCS, Ospedale San Raffaele, Milan, Italy
  3. Department of Engineering for Innovation, University of Salento, Italy
  4. IRCCS Istituto Ortopedico Galeazzi, Milan, Italy
  5. Department of Biomedical Sciences for Health, University of Milan, Italy

PMID: 29185298

Abstract

This study evaluated a tendon substitute model. Tenocytes were isolated from pig Achilles tendon, seeded onto scaffolds (Opocrin 2%, Typeone 3% and Symatese 2%) and studied by histology, immunofluorescence for collagen type 1 and 3 and biochemical analysis to assess cellularity. The permeability of these compounds was evaluated in the presence or absence of fibrin glue. Opocrin 2% was the best choice for cellular distribution within the scaffolds, which were then cultured for T0, T4, T7 and T10 days. Fibrin glue has been strongly supportive for the survival of cells with a significant increase in DNA content at T10 (P<0.05). Moreover, the synthetic activity of fibrin-free scaffolds was always negative. Lastly, a progressive increase in collagen 1 and 3 with fibrin-glue was observed. However, static culture is not sufficient to support long-term cellular activities and at T10 there is still a lack of organized matrix similar to the native tissue.

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