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Schiller A, Zhang T, Li R, et al. A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool. Cells. 2017;6(4)doi: 10.3390/cells6040047.
Schiller, A., Zhang, T., Li, R., Duechting, A., Sundararaman, S., Przybyla, A., Kuerten, S., & Lehmann, P. V. (2017). A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool. Cells, 6(4), . https://doi.org/10.3390/cells6040047
Schiller, Annemarie, et al. "A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool." Cells vol. 6,4 (2017). doi: https://doi.org/10.3390/cells6040047
Schiller A, Zhang T, Li R, Duechting A, Sundararaman S, Przybyla A, Kuerten S, Lehmann PV. A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool. Cells. 2017 Dec 07;6(4). doi: 10.3390/cells6040047. PMID: 29215584; PMCID: PMC5753071.
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Cells. 2017 Dec 07;6(4). doi: 10.3390/cells6040047.

A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool.

Cells

Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten, Paul V Lehmann

Affiliations

  1. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  2. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  3. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  4. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  5. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  6. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].
  7. Department of Cancer Immunology, Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-701 Poznan, Poland. [email protected].
  8. Institute of Anatomy and Cell Biology, Friedrich-Alexander University Erlangen-Nürnberg, 1054 Erlangen, Germany. [email protected].
  9. Research&Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. [email protected].

PMID: 29215584 PMCID: PMC5753071 DOI: 10.3390/cells6040047

Abstract

Testing of peripheral blood mononuclear cells (PBMC) for immune monitoring purposes requires verification of their functionality. This is of particular concern when the PBMC have been shipped or stored for prolonged periods of time. While the CEF (Cytomegalo-, Epstein-Barr and Flu-virus) peptide pool has become the gold standard for testing CD8 cell functionality, a positive control for CD4 cells is so far lacking. The latter ideally consists of proteins so as to control for the functionality of the antigen processing and presentation compartments, as well. Aiming to generate a positive control for CD4 cells, we first selected 12 protein antigens from infectious/environmental organisms that are ubiquitous: Varicella, Influenza, Parainfluenza, Mumps, Cytomegalovirus,

Keywords: CD4 cell function; ELISPOT; ImmunoSpot; PBMC cryopreservation; PBMC quality assessment; immune monitoring

Conflict of interest statement

P.V.L. is Founder, President and CEO of CTL, a company that specializes in immune monitoring by ELISPOT. T.Z. and R.L. are employees of CTL, A.S., A.D. and A.P. are visiting scholars at CTL. The autho

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