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Murashova GA, Mancuso CA, Canfield JL, et al. Multimodal nonlinear optical imaging of unstained retinas in the epi-direction with a sub-40 fs Yb-fiber laser. Biomed Opt Express. 2017;8(11):5228-5242doi: 10.1364/BOE.8.005228.
Murashova, G. A., Mancuso, C. A., Canfield, J. L., Sakami, S., Palczewski, K., Palczewska, G., & Dantus, M. (2017). Multimodal nonlinear optical imaging of unstained retinas in the epi-direction with a sub-40 fs Yb-fiber laser. Biomedical optics express, 8(11), 5228-5242. https://doi.org/10.1364/BOE.8.005228
Murashova, Gabrielle A, et al. "Multimodal nonlinear optical imaging of unstained retinas in the epi-direction with a sub-40 fs Yb-fiber laser." Biomedical optics express vol. 8,11 (2017): 5228-5242. doi: https://doi.org/10.1364/BOE.8.005228
Murashova GA, Mancuso CA, Canfield JL, Sakami S, Palczewski K, Palczewska G, Dantus M. Multimodal nonlinear optical imaging of unstained retinas in the epi-direction with a sub-40 fs Yb-fiber laser. Biomed Opt Express. 2017 Oct 26;8(11):5228-5242. doi: 10.1364/BOE.8.005228. eCollection 2017 Nov 01. PMID: 29188116; PMCID: PMC5695966.
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Biomed Opt Express. 2017 Oct 26;8(11):5228-5242. doi: 10.1364/BOE.8.005228. eCollection 2017 Nov 01.

Multimodal nonlinear optical imaging of unstained retinas in the epi-direction with a sub-40 fs Yb-fiber laser.

Biomedical optics express

Gabrielle A Murashova, Christopher A Mancuso, Jacob L Canfield, Sanae Sakami, Krzysztof Palczewski, Grazyna Palczewska, Marcos Dantus

Affiliations

  1. Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
  2. Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA.
  3. Department of Medical Devices, Polgenix Inc., Cleveland, OH 44106, USA.

PMID: 29188116 PMCID: PMC5695966 DOI: 10.1364/BOE.8.005228

Abstract

Ultrafast lasers have potential use in ophthalmology for diagnoses through non-invasive imaging as well as for surgical therapies or for evaluating pharmacological therapies. New ultrafast laser sources, operating at 1.07 μm and sub-40 fs pulse durations, offer exciting possibilities in multiphoton imagining of the retina as the bulk of the eye is relatively transparent to this wavelength, three-photon excitation is not absorbed by DNA, and this wavelength has a greater penetration depth compared to the commonly used 800 nm Ti:Sapphire laser. In this work, we present the first epi-direction detected cross-section and depth-resolved images of unstained isolated retinas obtained using multiphoton microscopy with an ultrafast fiber laser centered at 1.07 μm and a ~38 fs pulse duration. Spectral and temporal characterization of the autofluorescence signals show two distinct regions; the first one from the nerve fiber layer to the inner receptor layer, and the second being the retinal pigmented epithelium and choroid.

Keywords: (140.3510) Lasers, fiber; (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (320.7090) Ultrafast lasers

Conflict of interest statement

MD Biophotonic Solutions Inc. and IPG Photonics (C, P), GP Polgenix Inc. (I, E), KP Polgenix Inc. (I. S). All other authors declare that there are no conflicts of interest related to this article.

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