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Ge L, Cao C, Chen S, et al. Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction. Am J Transl Res. 2017;9(12):5289-5298
Ge, L., Cao, C., Chen, S., Shao, B., Li, Q., Li, Q., Liu, L., Liang, Z., & Huang, Y. (2017). Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction. American journal of translational research, 9(12), 5289-5298.
Ge, Liangpeng, et al. "Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction." American journal of translational research vol. 9,12 (2017): 5289-5298.
Ge L, Cao C, Chen S, Shao B, Li Q, Li Q, Liu L, Liang Z, Huang Y. Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction. Am J Transl Res. 2017 Dec 15;9(12):5289-5298. eCollection 2017. PMID: 29312483; PMCID: PMC5752881.
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Am J Transl Res. 2017 Dec 15;9(12):5289-5298. eCollection 2017.

Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction.

American journal of translational research

Liangpeng Ge, Chuan Cao, Shixuan Chen, Bin Shao, Qianyong Li, Qingtao Li, Lubin Liu, Zhiqing Liang, Yong Huang

Affiliations

  1. Chongqing Academy of Animal SciencesChongqing, China.
  2. Key Laboratory of Pig Industry Sciences, Ministry of AgricultureChongqing, China.
  3. Department of Plastic and Reconstructive Surgery, Southwestern Hospital, Third Military Medical UniversityChongqing 400038, China.
  4. Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical CenterOmaha, Nebraska 68198, United States.
  5. Department of Veterinary, Rongchang Campus, Southwest UniversityChongqing, China.
  6. School of Medicine, South China University of TechnologyChina.
  7. Chongqing Health Center for Women and Children, Chongqing Obstetric and Gynecologic HospitalChongqing, China.
  8. Key Laboratory of Pig Industry SciencesChongqing, China.

PMID: 29312483 PMCID: PMC5752881

Abstract

Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. A common strategy for POP reconstruction is to use scaffold materials to reconstruct the prolapsed pelvic organ. However, the existing materials for pelvic reconstruction do not meet the clinical requirements for biocompatibility, mechanics and immunological rejection. To address these concerns, urinary bladder decellularized materials (UBDM) was selected due to their good strain-stress resistance. To enhance its biocompatibility, laminin/nidogen was used to modify the UBDM with a mussel-inspired polydopamine coating. We found that the biocompatibility and mechanical properties of laminin/nidogen-Dopamine-UBDM were significantly enhanced and that the degradation rate of laminin/nidogen-Dopamine-UBDM was markedly reduced. Moreover, the expression of CD31 in the laminin/nidogen-Dopamine-UBDM group was higher than that in the normal UBDM group. The laminin/nidogen-Dopamine-UBDM treatment mainly guided M2 type macrophages and led to an inflammatory response. These results indicate that laminin/nidogen adsorbed urinary bladder decellularized materials are promising for use in pelvic reconstruction.

Keywords: Pelvic organ prolapse; decellularization; dopamine; laminin/nidogen; reconstruction; urinary bladder

Conflict of interest statement

None.

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