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Thakre PP, Bellingham MC. Capsaicin Enhances Glutamatergic Synaptic Transmission to Neonatal Rat Hypoglossal Motor Neurons via a TRPV1-Independent Mechanism. Front Cell Neurosci. 2017;11:383doi: 10.3389/fncel.2017.00383.
Thakre, P. P., & Bellingham, M. C. (2017). Capsaicin Enhances Glutamatergic Synaptic Transmission to Neonatal Rat Hypoglossal Motor Neurons via a TRPV1-Independent Mechanism. Frontiers in cellular neuroscience, 11383. https://doi.org/10.3389/fncel.2017.00383
Thakre, Prajwal P, and Bellingham, Mark C. "Capsaicin Enhances Glutamatergic Synaptic Transmission to Neonatal Rat Hypoglossal Motor Neurons via a TRPV1-Independent Mechanism." Frontiers in cellular neuroscience vol. 11 (2017): 383. doi: https://doi.org/10.3389/fncel.2017.00383
Thakre PP, Bellingham MC. Capsaicin Enhances Glutamatergic Synaptic Transmission to Neonatal Rat Hypoglossal Motor Neurons via a TRPV1-Independent Mechanism. Front Cell Neurosci. 2017 Dec 05;11:383. doi: 10.3389/fncel.2017.00383. eCollection 2017. PMID: 29259542; PMCID: PMC5723349.
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Front Cell Neurosci. 2017 Dec 05;11:383. doi: 10.3389/fncel.2017.00383. eCollection 2017.

Capsaicin Enhances Glutamatergic Synaptic Transmission to Neonatal Rat Hypoglossal Motor Neurons via a TRPV1-Independent Mechanism.

Frontiers in cellular neuroscience

Prajwal P Thakre, Mark C Bellingham

Affiliations

  1. Faculty of Medicine, School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia.

PMID: 29259542 PMCID: PMC5723349 DOI: 10.3389/fncel.2017.00383

Abstract

We investigated whether capsaicin modulated synaptic transmission to hypoglossal motor neurons (HMNs) by acting on transient receptor potential vanilloid type 1 (TRPV1) receptors. Using whole-cell patch clamp recording from neonatal rat HMNs, we found that capsaicin increased spontaneous excitatory post-synaptic current (sEPSC) frequency and amplitude. Interestingly, the only effect of capsaicin on spontaneous inhibitory post-synaptic currents (sIPSCs) was a significant decrease in sIPSC amplitude without altering frequency, indicating a post-synaptic mechanism of action. The frequency of miniature excitatory post-synaptic currents (mEPSCs), recorded in the presence of tetrodotoxin (TTX), was also increased by capsaicin, but capsaicin did not alter mEPSC amplitude, consistent with a pre-synaptic mechanism of action. A negative shift in membrane current (I

Keywords: TRPV1; capsaicin; excitatory synaptic transmission; hypoglossal motor neurons; inhibitory synaptic transmission; intrinsic excitability

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