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Backman L, Persson K. The No-Nonsens SDS-PAGE. Methods Mol Biol. 2018;1721:89-94doi: 10.1007/978-1-4939-7546-4_8.
Backman, L., & Persson, K. (2018). The No-Nonsens SDS-PAGE. Methods in molecular biology (Clifton, N.J.), 172189-94. https://doi.org/10.1007/978-1-4939-7546-4_8
Backman, Lars, and Persson, Karina. "The No-Nonsens SDS-PAGE." Methods in molecular biology (Clifton, N.J.) vol. 1721 (2018): 89-94. doi: https://doi.org/10.1007/978-1-4939-7546-4_8
Backman L, Persson K. The No-Nonsens SDS-PAGE. Methods Mol Biol. 2018;1721:89-94. doi: 10.1007/978-1-4939-7546-4_8. PMID: 29423849.
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Methods Mol Biol. 2018;1721:89-94. doi: 10.1007/978-1-4939-7546-4_8.

The No-Nonsens SDS-PAGE.

Methods in molecular biology (Clifton, N.J.)

Lars Backman, Karina Persson

Affiliations

  1. Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden. [email protected].
  2. Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.

PMID: 29423849 DOI: 10.1007/978-1-4939-7546-4_8

Abstract

The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680-685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

Keywords: SDS-PAGE; polyacrylamide gel electrophoresis

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