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Rennie MY, Stovall S, Carson JP, et al. Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract. J Cardiovasc Dev Dis. 2017;4(4)doi: 10.3390/jcdd4040024.
Rennie, M. Y., Stovall, S., Carson, J. P., Danilchik, M., Thornburg, K. L., & Rugonyi, S. (2017). Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract. Journal of cardiovascular development and disease, 4(4), . https://doi.org/10.3390/jcdd4040024
Rennie, Monique Y, et al. "Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract." Journal of cardiovascular development and disease vol. 4,4 (2017). doi: https://doi.org/10.3390/jcdd4040024
Rennie MY, Stovall S, Carson JP, Danilchik M, Thornburg KL, Rugonyi S. Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract. J Cardiovasc Dev Dis. 2017 Dec 20;4(4). doi: 10.3390/jcdd4040024. PMID: 29367553; PMCID: PMC5753125.
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J Cardiovasc Dev Dis. 2017 Dec 20;4(4). doi: 10.3390/jcdd4040024.

Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract.

Journal of cardiovascular development and disease

Monique Y Rennie, Stephanie Stovall, James P Carson, Michael Danilchik, Kent L Thornburg, Sandra Rugonyi

Affiliations

  1. Knight Cardiovascular Institute, Center for Developmental Health, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].
  2. Biomedical Engineering, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].
  3. Texas Advanced Computing Center, University of Texas, Austin, TX 78758, USA. [email protected].
  4. Integrative Biosciences, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].
  5. Knight Cardiovascular Institute, Center for Developmental Health, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].
  6. Knight Cardiovascular Institute, Center for Developmental Health, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].
  7. Biomedical Engineering, Oregon Health & Science University, Portland, OR 97239, USA. [email protected].

PMID: 29367553 PMCID: PMC5753125 DOI: 10.3390/jcdd4040024

Abstract

Blood flow is critical for normal cardiac development. Hemodynamic stimuli outside of normal ranges can lead to overt cardiac defects, but how early heart tissue remodels in response to altered hemodynamics is poorly understood. This study investigated changes in tissue collagen in response to hemodynamic overload in the chicken embryonic heart outflow tract (OFT) during tubular heart stages (HH18 to HH24, ~24 h). A suture tied around the OFT at HH18 was tightened to constrict the lumen for ~24 h (constriction range at HH24: 15-60%). Expression of fibril collagens I and III and fibril organizing collagens VI and XIV were quantified at the gene and protein levels via qPCR and quantitative immunofluorescence. Collagen I was slightly elevated upstream of the band and in the cushions in banded versus control OFTs. Changes in collagen III were not observed. Collagen VI deposition was elevated downstream of the band, but not overall. Collagen XIV deposition increased throughout the OFT, and strongly correlated to lumen constriction. Interestingly, organization of collagen I fibrils was observed for the tighter banded embryos in regions that also showed increase in collagen XIV deposition, suggesting a potentially key role for collagens I and XIV in the structural adaptation of embryonic heart tissue to hemodynamic overload.

Keywords: cardiac development; congenital heart disease; heart malformation; mechanotransduction; tissue remodeling

Conflict of interest statement

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and

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