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Villalta D, D'Aurizio F, Da Re M, et al. Diagnostic accuracy of a new fluoroenzyme immunoassay for the detection of TSH receptor autoantibodies in Graves' disease. Auto Immun Highlights. 2018;9(1):3doi: 10.1007/s13317-018-0102-4.
Villalta, D., D'Aurizio, F., Da Re, M., Ricci, D., Latrofa, F., & Tozzoli, R. (2018). Diagnostic accuracy of a new fluoroenzyme immunoassay for the detection of TSH receptor autoantibodies in Graves' disease. Auto- immunity highlights, 9(1), 3. https://doi.org/10.1007/s13317-018-0102-4
Villalta, Danilo, et al. "Diagnostic accuracy of a new fluoroenzyme immunoassay for the detection of TSH receptor autoantibodies in Graves' disease." Auto- immunity highlights vol. 9,1 (2018): 3. doi: https://doi.org/10.1007/s13317-018-0102-4
Villalta D, D'Aurizio F, Da Re M, Ricci D, Latrofa F, Tozzoli R. Diagnostic accuracy of a new fluoroenzyme immunoassay for the detection of TSH receptor autoantibodies in Graves' disease. Auto Immun Highlights. 2018 Feb 12;9(1):3. doi: 10.1007/s13317-018-0102-4. PMID: 29435670; PMCID: PMC5809681.
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Auto Immun Highlights. 2018 Feb 12;9(1):3. doi: 10.1007/s13317-018-0102-4.

Diagnostic accuracy of a new fluoroenzyme immunoassay for the detection of TSH receptor autoantibodies in Graves' disease.

Auto- immunity highlights

Danilo Villalta, Federica D'Aurizio, Mirella Da Re, Debora Ricci, Francesco Latrofa, Renato Tozzoli

Affiliations

  1. Immunology and Allergy Unit, S. Maria degli Angeli Hospital, Pordenone, Italy. [email protected].
  2. Clinical Pathology Laboratory, University Hospital, Udine, Italy.
  3. Immunology and Allergy Unit, S. Maria degli Angeli Hospital, Pordenone, Italy.
  4. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
  5. Clinical Pathology Laboratory, S. Maria degli Angeli Hospital, Pordenone, Italy.

PMID: 29435670 PMCID: PMC5809681 DOI: 10.1007/s13317-018-0102-4

Abstract

PURPOSE: Thyrotropin receptor (TSHR) autoantibodies (TRAbs) are a hallmark of Graves' disease (GD). The aim of this study was to evaluate the diagnostic accuracy of a new third generation automatic fluorescence enzyme immunoassay for TRAb measurement in GD, in comparison with two current IMAs.

METHODS: Sera of 439 subjects (57 patients with untreated GD, 34 with treated GD, 15 with GD and Graves' orbitopathy, 52 with multinodular non-toxic goiter, 86 with Hashimoto's thyroiditis, 20 with toxic adenoma or toxic multinodular goiter, 55 with non-thyroid autoimmune diseases and 120 normal controls) were tested for TRAbs with the ELiA

RESULTS: Sensitivity and specificity of the ELiA™ anti-TSH-R assay, TRAK™ RIA and Immulite™ TSI assay were 94.7% and 99.6, 100 and 98.2%, 100 and 98.2%, respectively. Spearman's coefficient and Passing-Bablok regression showed a satisfactory correlation between EliA™ and TRAK™ [rho: 0.925; 95% CI: 0.883-0-953. Intercept: - 0.875 (95% CI: - 2.411 to 0.194); slope: 1.086 (95% CI: 0.941 to 1.248)], and between ELiA

CONCLUSIONS: The diagnostic performance of ELiA

Keywords: ELiA™ anti-TSH-R assay; Graves’ disease (GD); Immunometric assays; Thyrotropin receptor autoantibodies (TRAbs)

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