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Rojas-Canales DM, Waibel M, Forget A, et al. Oxygen-permeable microwell device maintains islet mass and integrity during shipping. Endocr Connect. 2018;7(3):490-503doi: 10.1530/EC-17-0349.
Rojas-Canales, D. M., Waibel, M., Forget, A., Penko, D., Nitschke, J., Harding, F. J., Delalat, B., Blencowe, A., Loudovaris, T., Grey, S. T., Thomas, H. E., Kay, T. W. H., Drogemuller, C. J., Voelcker, N. H., & Coates, P. T. (2018). Oxygen-permeable microwell device maintains islet mass and integrity during shipping. Endocrine connections, 7(3), 490-503. https://doi.org/10.1530/EC-17-0349
Rojas-Canales, Darling M, et al. "Oxygen-permeable microwell device maintains islet mass and integrity during shipping." Endocrine connections vol. 7,3 (2018): 490-503. doi: https://doi.org/10.1530/EC-17-0349
Rojas-Canales DM, Waibel M, Forget A, Penko D, Nitschke J, Harding FJ, Delalat B, Blencowe A, Loudovaris T, Grey ST, Thomas HE, Kay TWH, Drogemuller CJ, Voelcker NH, Coates PT. Oxygen-permeable microwell device maintains islet mass and integrity during shipping. Endocr Connect. 2018 Mar;7(3):490-503. doi: 10.1530/EC-17-0349. Epub 2018 Feb 26. PMID: 29483160; PMCID: PMC5861371.
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Endocr Connect. 2018 Mar;7(3):490-503. doi: 10.1530/EC-17-0349. Epub 2018 Feb 26.

Oxygen-permeable microwell device maintains islet mass and integrity during shipping.

Endocrine connections

Darling M Rojas-Canales, Michaela Waibel, Aurelien Forget, Daniella Penko, Jodie Nitschke, Fran J Harding, Bahman Delalat, Anton Blencowe, Thomas Loudovaris, Shane T Grey, Helen E Thomas, Thomas W H Kay, Chris J Drogemuller, Nicolas H Voelcker, Patrick T Coates

Affiliations

  1. The Centre for Clinical and Experimental Transplantation (CCET) The Royal Adelaide HospitalAdelaide, South Australia, Australia.
  2. Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM)Adelaide, South Australia, Australia.
  3. Department of MedicineFaculty of Health and Medical Sciences, University of Adelaide, South Australia, Australia.
  4. St Vincent's Institute of Medical ResearchFitzroy, Victoria, Australia.
  5. The University of MelbourneDepartment of Medicine, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
  6. Science and Engineering FacultyQueensland University of Technology, Brisbane, Queensland, Australia.
  7. Future Industries InstituteUniversity of South Australia, Mawson Lakes, South Australia, Australia.
  8. School of Pharmacy and Medical SciencesUniversity of South Australia, Adelaide, South Australia, Australia.
  9. Transplantation Immunology GroupGarvan Institute of Medical Research, Darlinghurst, New South Wales, Australia.
  10. Monash Institute of Pharmaceutical SciencesMonash University, Parkville, Victoria, Australia.
  11. The Centre for Clinical and Experimental Transplantation (CCET) The Royal Adelaide HospitalAdelaide, South Australia, Australia [email protected].

PMID: 29483160 PMCID: PMC5861371 DOI: 10.1530/EC-17-0349

Abstract

Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20-40% of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.

© 2018 The authors.

Keywords: hypoxia; islet; microwell; shipping; transplantation

References

  1. Biomaterials. 2014 Jul;35(23):6060-8 - PubMed
  2. Transplant Proc. 2005 Oct;37(8):3407-8 - PubMed
  3. Transplant Proc. 2014 Jul-Aug;46(6):1989-91 - PubMed
  4. Horm Metab Res. 2015 Jan;47(1):16-23 - PubMed
  5. Biotechnol Prog. 2014 Jan-Feb;30(1):178-87 - PubMed
  6. Am J Physiol Endocrinol Metab. 2006 May;290(5):E771-9 - PubMed
  7. Transplant Proc. 2008 Mar;40(2):395-400 - PubMed
  8. Transpl Int. 2008 Nov;21(11):1029-35 - PubMed
  9. Cell Med. 2011 Dec 09;2(3):97-104 - PubMed
  10. Am J Transplant. 2007 Apr;7(4):1010-20 - PubMed
  11. N Engl J Med. 2006 Sep 28;355(13):1318-30 - PubMed
  12. Cell Transplant. 2013;22(11):2147-59 - PubMed
  13. Cell Transplant. 2012;21(9):2063-78 - PubMed
  14. PLoS One. 2012;7(1):e30415 - PubMed
  15. Diabetes Care. 2016 Jul;39(7):1230-40 - PubMed
  16. Acta Biomater. 2012 Feb;8(2):608-18 - PubMed
  17. Diabetes. 2007 Mar;56(3):594-603 - PubMed
  18. Islets. 2010 Mar-Apr;2(2):89-95 - PubMed
  19. Am J Transplant. 2014 Nov;14(11):2595-606 - PubMed
  20. Biomed Res Int. 2013;2013:975608 - PubMed
  21. Biomaterials. 2013 Mar;34(10):2463-71 - PubMed
  22. Diabetes Care. 2004 Apr;27(4):895-900 - PubMed
  23. ACS Appl Mater Interfaces. 2014 Jun 11;6(11):8090-7 - PubMed
  24. J Clin Endocrinol Metab. 2008 Mar;93(3):1046-53 - PubMed
  25. Cell Transplant. 2013;22(2):253-66 - PubMed
  26. ACS Appl Mater Interfaces. 2016 Feb;8(7):4467-76 - PubMed
  27. Islets. 2012 Mar-Apr;4(2):152-7 - PubMed
  28. Horm Metab Res. 1996 Jun;28(6):287-8 - PubMed
  29. Eur J Immunol. 2012 Jul;42(7):1717-22 - PubMed
  30. Tissue Eng Part C Methods. 2012 Aug;18(8):583-92 - PubMed
  31. Curr Opin Organ Transplant. 2011 Dec;16(6):627-31 - PubMed
  32. Transplant Proc. 2014 Jul-Aug;46(6):1985-8 - PubMed
  33. Transplant Proc. 2013 Oct;45(8):3097-101 - PubMed
  34. Diabetes. 1988 Apr;37(4):413-20 - PubMed
  35. Int J Endocrinol. 2014;2014:451035 - PubMed
  36. Transplantation. 2010 Jan 15;89(1):47-54 - PubMed
  37. N Engl J Med. 2000 Jul 27;343(4):230-8 - PubMed
  38. Diabetologia. 2015 Feb;58(2):221-32 - PubMed
  39. Islets. 2011 Jan-Feb;3(1):1-5 - PubMed
  40. Ann Biomed Eng. 2013 Mar;41(3):469-76 - PubMed
  41. J Clin Invest. 1992 Jul;90(1):77-85 - PubMed
  42. N Engl J Med. 1993 Sep 30;329(14 ):977-86 - PubMed
  43. Diabetes. 1999 Jul;48(7):1402-8 - PubMed
  44. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84 - PubMed
  45. Biochem J. 2015 Mar 1;466(2):203-18 - PubMed
  46. Am J Transplant. 2007 Mar;7(3):707-13 - PubMed
  47. Biotechnol Bioeng. 2007 Dec 1;98(5):1071-82 - PubMed
  48. Cell Transplant. 2004;13(5):481-8 - PubMed
  49. Lancet. 2014 Jan 4;383(9911):69-82 - PubMed
  50. Diabetes Obes Metab. 2010 Oct;12 Suppl 2:159-67 - PubMed
  51. Biomater Sci. 2017 Mar 28;5(4):828-836 - PubMed
  52. Biomaterials. 2014 Jun;35(17):4815-26 - PubMed
  53. J Cell Mol Med. 2015 Aug;19(8):1836-46 - PubMed
  54. Curr Opin Organ Transplant. 2008 Aug;13(4):445-51 - PubMed
  55. Am J Transplant. 2013 Jul;13(7):1850-8 - PubMed
  56. Endocr Rev. 1994 Aug;15(4):516-42 - PubMed
  57. Pediatr Clin North Am. 2005 Dec;52(6):1553-78 - PubMed
  58. PLoS One. 2013 May 30;8(5):e64772 - PubMed
  59. Arq Bras Endocrinol Metabol. 2008 Mar;52(2):146-55 - PubMed
  60. Cell Death Dis. 2012 Jun 14;3:e322 - PubMed

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