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Front Plant Sci. 2018 Feb 01;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017.

A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of Root Colonizing Pseudomonads.

Frontiers in plant science

Rosemarie Wilton, Angela J Ahrendt, Shalaka Shinde, Deirdre J Sholto-Douglas, Jessica L Johnson, Melissa B Brennan, Kenneth M Kemner

Affiliations

  1. Biosciences Division, Argonne National Laboratory, Argonne, IL, United States.
  2. Center for Synchrotron Radiation Research and Instrumentation, Illinois Institute of Technology, Chicago, IL, United States.

PMID: 29449848 PMCID: PMC5799272 DOI: 10.3389/fpls.2017.02242

Abstract

In the terrestrial ecosystem, plant-microbe symbiotic associations are ecologically and economically important processes. To better understand these associations at structural and functional levels, different molecular and biochemical tools are applied. In this study, we have constructed a suite of vectors that incorporates several new elements into the rhizosphere stable, broad-host vector pME6031. The new vectors are useful for studies requiring multi-color tagging and visualization of plant-associated, Gram-negative bacterial strains such as

Keywords: Pseudomonas; biocontrol strain; broad-host vector; fluorescent protein; pVS1; plant growth promotion; rhizosphere

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