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Cazaña-Pérez V, Cidad P, Donate-Correa J, et al. Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum. Front Physiol. 2018;9:89doi: 10.3389/fphys.2018.00089.
Cazaña-Pérez, V., Cidad, P., Donate-Correa, J., Martín-Núñez, E., López-López, J. R., Pérez-García, M. T., Giraldez, T., Navarro-González, J. F., & Alvarez de la Rosa, D. (2018). Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum. Frontiers in physiology, 989. https://doi.org/10.3389/fphys.2018.00089
Cazaña-Pérez, Violeta, et al. "Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum." Frontiers in physiology vol. 9 (2018): 89. doi: https://doi.org/10.3389/fphys.2018.00089
Cazaña-Pérez V, Cidad P, Donate-Correa J, Martín-Núñez E, López-López JR, Pérez-García MT, Giraldez T, Navarro-González JF, Alvarez de la Rosa D. Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum. Front Physiol. 2018 Feb 12;9:89. doi: 10.3389/fphys.2018.00089. eCollection 2018. PMID: 29483881; PMCID: PMC5816230.
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Front Physiol. 2018 Feb 12;9:89. doi: 10.3389/fphys.2018.00089. eCollection 2018.

Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum.

Frontiers in physiology

Violeta Cazaña-Pérez, Pilar Cidad, Javier Donate-Correa, Ernesto Martín-Núñez, José R López-López, M Teresa Pérez-García, Teresa Giraldez, Juan F Navarro-González, Diego Alvarez de la Rosa

Affiliations

  1. Departamento de Ciencias Médicas Básicas (Fisiología), Instituto de Tecnologías Biomédicas and Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna, Tenerife, Spain.
  2. Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Tenerife, Spain.
  3. Departamento de Bioquímica y Biología Molecular y Fisiología e Instituto de Biología y Genética Molecular (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain.

PMID: 29483881 PMCID: PMC5816230 DOI: 10.3389/fphys.2018.00089

Abstract

Patients with chronic kidney disease (CKD) have a markedly increased incidence of cardiovascular disease (CVD). The high concentration of circulating uremic toxins and alterations in mineral metabolism and hormone levels produce vascular wall remodeling and significant vascular damage. Medial calcification is an early vascular event in CKD patients and is associated to apoptosis or necrosis and trans-differentiation of vascular smooth muscle cells (VSMC) to an osteogenic phenotype. VSMC obtained from bovine or rat aorta and cultured in the presence of increased inorganic phosphate (Pi) have been extensively used to study these processes. In this study we used human aortic VSMC primary cultures to compare the effects of increased Pi to treatment with serum obtained from uremic patients. Uremic serum induced calcification, trans-differentiation and phenotypic remodeling even with normal Pi levels. In spite of similar calcification kinetics, there were fundamental differences in osteochondrogenic marker expression and alkaline phosphatase induction between Pi and uremic serum-treated cells. Moreover, high Pi induced a dramatic decrease in cell viability, while uremic serum preserved it. In summary, our data suggests that primary cultures of human VSMC treated with serum from uremic patients provides a more informative model for the study of vascular calcification secondary to CKD.

Keywords: apoptosis; chronic kidney disease; human aorta; inorganic phosphate; osteogenic differentiation; uremia; uremic serum; vascular calcification

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