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Zerbinati N, Lotti T, Monticelli D, et al. . Open Access Maced J Med Sci. 2018;6(1):15-19doi: 10.3889/oamjms.2018.044.
Zerbinati, N., Lotti, T., Monticelli, D., Rauso, R., González-Isaza, P., D'Este, E., Calligaro, A., Sommatis, S., Maccario, C., Mocchi, R., Lotti, J., Wollina, U., Tchernev, G., & França, K. (2018). . Open access Macedonian journal of medical sciences, 6(1), 15-19. https://doi.org/10.3889/oamjms.2018.044
Zerbinati, Nicola, et al. "." Open access Macedonian journal of medical sciences vol. 6,1 (2018): 15-19. doi: https://doi.org/10.3889/oamjms.2018.044
Zerbinati N, Lotti T, Monticelli D, Rauso R, González-Isaza P, D'Este E, Calligaro A, Sommatis S, Maccario C, Mocchi R, Lotti J, Wollina U, Tchernev G, França K. . Open Access Maced J Med Sci. 2018 Jan 07;6(1):15-19. doi: 10.3889/oamjms.2018.044. eCollection 2018 Jan 25. PMID: 29483971; PMCID: PMC5816290.
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Open Access Maced J Med Sci. 2018 Jan 07;6(1):15-19. doi: 10.3889/oamjms.2018.044. eCollection 2018 Jan 25.

[No title available]

Open access Macedonian journal of medical sciences

Nicola Zerbinati, Torello Lotti, Damiano Monticelli, Raffaele Rauso, Pablo González-Isaza, Edoardo D'Este, Alberto Calligaro, Sabrina Sommatis, Cristina Maccario, Roberto Mocchi, Jacopo Lotti, Uwe Wollina, Georgi Tchernev, Katlein França

Affiliations

  1. Universita degli Studi dell'Insubria Dipartimento di Scienze Chirurgiche e Morfologiche, Varese, Italy.
  2. University of Rome "G. Marconi" - Centro Studi per la Ricerca Multidisciplinare e Rigenerativa (CSRMR), Rome, Italy.
  3. Universita degli Studi dell'Insubria Dipartimento di Scienza e Alta Tecnologia, Como, Italy.
  4. University of Foggia - Department of Plastic Reconstructive Surgery, Foggia, Italy.
  5. Hospital Universitario San Jorge - Head Chief of Urogynecology, Carrera 32410, Pereira, Risaralda 660002, Colombia.
  6. Centro Medico Polispecialistico, Pavia, Italy.
  7. University of Pavia - Department of Public Health, Experimental and Forensic Medicine, Pavia, Italy.
  8. UB-CARE S.r.l. - Spin-off University of Pavia, Pavia, Italy.
  9. University of Rome "G. Marconi" - Department of Nuclear, Subnuclear and Radiation Physics, Rome, Italy.
  10. Städtisches Klinikum Dresden, Department of Dermatology and Allergology, 01067 Dresden, Germany.
  11. Department of Dermatology, Venereology and Dermatologic Surgery, Medical Institute of Ministry of Interior (MVR-Sofia), General Skobelev 79, 1606 Sofia, Bulgaria.
  12. Onkoderma - Policlinic for Dermatology, Venereology and Dermatologic Surgery, General Skobelev 26, 1606, Sofia, Bulgaria.
  13. University of Miami School of Medicine, 1400 NW 10th Avenue, Miami, Florida 33136-1015, United States.

PMID: 29483971 PMCID: PMC5816290 DOI: 10.3889/oamjms.2018.044

Abstract

OBJECTIVE: Neauvia Stimulate is biocompatible, injectable hyaluronic acid (HA) filler (26 mg/ml) PEG cross-linked with 1% of calcium hydroxyapatite (CaHA) for facial soft-tissue augmentation that provides volume to tissues, followed by process of neocollagenesis for improving skin quality.

AIM: The aim of the present study is to evaluate the biosafety of the product (Lot. 160517-26-1/2 PEG) on human keratinocytes cultured in vitro.

MATERIAL AND METHODS: The experimental model proposed, despite being an in vitro system, allows the derivation of useful information to predict the possible activity of the product in further in vivo application. Human keratinocytes (HaCaT cells) were treated with the product for 24h at increasing concentrations of product respect to control (untreated cells).

RESULTS: The biosafety of the product to be tested has been evaluated performing different methods: MTT test, NRU test, Kenacid Blue assay. Moreover, any possible effect on the structure, morphology, and viability of cells has been evaluated.

CONCLUSION: In conclusion, the results obtained by the different methods show that the product Neauvia Stimulate® does not cause any cytotoxic effect and does not affect the correct structure and morphology of cells cultures.

Keywords: Neauvia Stimulate; biosafety; calcium hydroxyapatite; human keratinocytes; hyaluronic acid

References

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