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Bubnov RV, Drahulian MV, Buchek PV, et al. High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model. EPMA J. 2017;9(1):59-75doi: 10.1007/s13167-017-0115-5.
Bubnov, R. V., Drahulian, M. V., Buchek, P. V., & Gulko, T. P. (2018). High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model. The EPMA journal, 9(1), 59-75. https://doi.org/10.1007/s13167-017-0115-5
Bubnov, Rostyslav V, et al. "High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model." The EPMA journal vol. 9,1 (2018): 59-75. doi: https://doi.org/10.1007/s13167-017-0115-5
Bubnov RV, Drahulian MV, Buchek PV, Gulko TP. High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model. EPMA J. 2017 Oct 11;9(1):59-75. doi: 10.1007/s13167-017-0115-5. eCollection 2018 Mar. PMID: 29515688; PMCID: PMC5833895.
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EPMA J. 2017 Oct 11;9(1):59-75. doi: 10.1007/s13167-017-0115-5. eCollection 2018 Mar.

High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model.

The EPMA journal

Rostyslav V Bubnov, Maria V Drahulian, Polina V Buchek, Tamara P Gulko

Affiliations

  1. 1Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Zabolotny Str., 154, Kyiv, 03143 Ukraine.
  2. Clinical Hospital 'Pheophania' of State Affairs Department, Zabolotny str., 21, Kyiv, 03143 Ukraine.
  3. 3Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotny str., 150, Kyiv, 03143 Ukraine.

PMID: 29515688 PMCID: PMC5833895 DOI: 10.1007/s13167-017-0115-5

Abstract

BACKGROUND: Liver fibrosis (LF) is a chronic disease, associated with many collateral diseases including reproductive dysfunction. Although the normal liver has a large regenerative capacity the complications of LF could be severe and irreversible. Hormone and sex-related issues of LF development and interactions with male reproductive have not been finally studied. The aim was to study the reproductive function of male rats in experimental CCl

MATERIALS: Studies were conducted on 20 3-month old Wistar male rats. The experimental animals were injected with freshly prepared 50% olive oil solution of carbohydrate tetrachloride (CCl

RESULTS: On the 8th week of experiment we observed ultrasound manifestation of advanced liver fibrosis, including hepatosplenomegaly, portal hypertension. Ultrasound exam of the rat testes showed testicular degeneration, hydrocele, fibrosis, scarring, petrifications, size reduction, and restriction of testicular descent; testes size decreased from 1.24 ± 0.62 ml to 0.61 ± 0.13,

CONCLUSION: Carbohydrate tetrachloride induces injury of liver parenchyma evoking fast and severe liver fibrosis, and is associated with irreversible structural and functional changes in testes, reducing fertility, decreasing potential pregnancy rate, and affecting its development. Liver showed high potential to regenerate, however the self-restoring after liver fibrosis was not accompanied with recovery of the reproductive system.

Keywords: Animal model; Fertility; Liver fibrosis; Liver regeneration; Male reproductive system; Men health; Predictive preventive personalized medicine; Translation; Ultrasound; Wistar rats

Conflict of interest statement

Compliance with ethical standardsNot applicable.Authors declare that they have no competing interests.

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