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Comtet-Marre S, Chaucheyras-Durand F, Bouzid O, et al. FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota. Front Microbiol. 2018;9:215doi: 10.3389/fmicb.2018.00215.
Comtet-Marre, S., Chaucheyras-Durand, F., Bouzid, O., Mosoni, P., Bayat, A. R., Peyret, P., & Forano, E. (2018). FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota. Frontiers in microbiology, 9215. https://doi.org/10.3389/fmicb.2018.00215
Comtet-Marre, Sophie, et al. "FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota." Frontiers in microbiology vol. 9 (2018): 215. doi: https://doi.org/10.3389/fmicb.2018.00215
Comtet-Marre S, Chaucheyras-Durand F, Bouzid O, Mosoni P, Bayat AR, Peyret P, Forano E. FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota. Front Microbiol. 2018 Feb 13;9:215. doi: 10.3389/fmicb.2018.00215. eCollection 2018. PMID: 29487591; PMCID: PMC5816793.
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Front Microbiol. 2018 Feb 13;9:215. doi: 10.3389/fmicb.2018.00215. eCollection 2018.

FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota.

Frontiers in microbiology

Sophie Comtet-Marre, Frédérique Chaucheyras-Durand, Ourdia Bouzid, Pascale Mosoni, Ali R Bayat, Pierre Peyret, Evelyne Forano

Affiliations

  1. UMR 454 MEDIS, INRA, Université Clermont Auvergne, Clermont-Ferrand, France.
  2. R&D Animal Nutrition, Lallemand, Blagnac, France.
  3. Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Helsinki, Finland.

PMID: 29487591 PMCID: PMC5816793 DOI: 10.3389/fmicb.2018.00215

Abstract

Ruminants fulfill their energy needs for growth primarily through microbial breakdown of plant biomass in the rumen. Several biotic and abiotic factors influence the efficiency of fiber degradation, which can ultimately impact animal productivity and health. To provide more insight into mechanisms involved in the modulation of fibrolytic activity, a functional DNA microarray targeting genes encoding key enzymes involved in cellulose and hemicellulose degradation by rumen microbiota was designed. Eight carbohydrate-active enzyme (CAZyme) families (GH5, GH9, GH10, GH11, GH43, GH48, CE1, and CE6) were selected which represented 392 genes from bacteria, protozoa, and fungi. The DNA microarray, designated as FibroChip, was validated using targets of increasing complexity and demonstrated sensitivity and specificity. In addition, FibroChip was evaluated for its explorative and semi-quantitative potential. Differential expression of CAZyme genes was evidenced in the rumen bacterium

Keywords: carbohydrate esterases; cellulolysis; functional DNA microarray; glycoside hydrolases; hemicellulolysis; metatranscriptomics; rumen

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