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Chen L, Liu GQ, Wu HY, et al. Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis . Int J Ophthalmol. 2018;11(2):216-222doi: 10.18240/ijo.2018.02.06.
Chen, L., Liu, G. Q., Wu, H. Y., Jin, J., Yin, X., Li, D., & Lu, P. R. (2018). Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis . International journal of ophthalmology, 11(2), 216-222. https://doi.org/10.18240/ijo.2018.02.06
Chen, Lei, et al. "Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis ." International journal of ophthalmology vol. 11,2 (2018): 216-222. doi: https://doi.org/10.18240/ijo.2018.02.06
Chen L, Liu GQ, Wu HY, Jin J, Yin X, Li D, Lu PR. Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis . Int J Ophthalmol. 2018 Feb 18;11(2):216-222. doi: 10.18240/ijo.2018.02.06. eCollection 2018. PMID: 29487809; PMCID: PMC5824074.
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Int J Ophthalmol. 2018 Feb 18;11(2):216-222. doi: 10.18240/ijo.2018.02.06. eCollection 2018.

Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis .

International journal of ophthalmology

Lei Chen, Gao-Qin Liu, Hong-Ya Wu, Ji Jin, Xue Yin, Dan Li, Pei-Rong Lu

Affiliations

  1. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
  2. Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou 215006, Jiangsu Province, China.

PMID: 29487809 PMCID: PMC5824074 DOI: 10.18240/ijo.2018.02.06

Abstract

AIM: To explore the interaction between macrophages and chemokines [monocyte chemoattractant protein 1 (MCP-1/CCL2) and fractalkine/CX3CL1] and the effects of their interaction on neovascularization.

METHODS: Human peripheral blood mononuclear cells, donated by healthy volunteers, were separated and cultured in RPMI-1640 medium containing 10% fetal bovine serum, then induced into macrophages by stimulation with 30 µg/L granulocyte macrophage-colony stimulating factor (GM-CSF). The expression of CCR2 and/or CX3CR1 in the macrophages was examined using flow cytometry. Macrophages were then stimulated with recombinant human CCL2 (rh-CCL2) or recombinant human CX3CL1 (rh-CX3CL1). The expression of angiogenesis-related genes, including

RESULTS: The expression rate of CCR2 in macrophages stimulated by GM-CSF was 42%±1.9%. The expression rate of CX3CR1 was 71%±3.3%. Compared with vehicle-treated groups, gene expression of

CONCLUSION: CCL2 and CX3CL1 exert different effects in regulation of macrophage in expression of angiogenesis-related factors, including

Keywords: angiogenesis; chemokine; macrophage; migration; proliferation

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