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Morohoshi K, Mochinaga R, Watanabe T, et al. 16 kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis. Endocr Connect. 2018;7(5):630-636doi: 10.1530/EC-18-0116.
Morohoshi, K., Mochinaga, R., Watanabe, T., Nakajima, R., & Harigaya, T. (2018). 16 kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis. Endocrine connections, 7(5), 630-636. https://doi.org/10.1530/EC-18-0116
Morohoshi, Kazunori, et al. "16 kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis." Endocrine connections vol. 7,5 (2018): 630-636. doi: https://doi.org/10.1530/EC-18-0116
Morohoshi K, Mochinaga R, Watanabe T, Nakajima R, Harigaya T. 16 kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis. Endocr Connect. 2018 May;7(5):630-636. doi: 10.1530/EC-18-0116. Epub 2018 Apr 05. PMID: 29622663; PMCID: PMC5919937.
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Endocr Connect. 2018 May;7(5):630-636. doi: 10.1530/EC-18-0116. Epub 2018 Apr 05.

16 kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis.

Endocrine connections

Kazunori Morohoshi, Ryo Mochinaga, Tsukasa Watanabe, Ryojun Nakajima, Toshio Harigaya

Affiliations

  1. Department of Life SciencesLaboratory of Functional Anatomy, Faculty of Agriculture, Meiji University, Kawasaki, Japan [email protected].
  2. Department of Life SciencesLaboratory of Functional Anatomy, Faculty of Agriculture, Meiji University, Kawasaki, Japan.

PMID: 29622663 PMCID: PMC5919937 DOI: 10.1530/EC-18-0116

Abstract

Many functions of vasoinhibins have been reported, but its receptor has not been clarified yet. Vasoinhibins, 11-18 kDa N-terminal fragments of prolactin, have anti-angiogenic activity and act on endothelial cells to induce apoptosis and to inhibit migration and proliferation, which are opposite to the effects of prolactin. Although vasoinhibins bind to the prolactin receptor, its binding activity is very weak compared to prolactin. Therefore, in this study, we evaluated the binding activity between 16 kDa vasoinhibin and integrin beta1, alpha5 beta1, alpha1 beta1 and alphaV beta3 to identify a specific receptor for vasoinhibins. Moreover, we examined whether 16 kDa vasoinhibin induced apoptosis through integrin beta1 and alpha5 beta1 in endothelial cells. In this study, binding assays and co-immunoprecipitation experiments demonstrated that 16 kDa vasoinhibin could bind strongly to integrin beta1 and alpha5 beta1. Moreover, neutralizing with integrin beta1 and alpha5 beta1 antibody could inhibit 16 kDa vasoinhibin-induced apoptosis in endothelial cells. These findings suggest that vasoinhibins can act on endothelial cells through integrin alpha5 beta1 to induce apoptosis.

© 2018 The authors.

Keywords: 16 k prolactin; integrin; integrin alpha5 beta1; integrin beta1; vasoinhibins

References

  1. J Biol Chem. 1999 Apr 23;274(17):11721-6 - PubMed
  2. Am J Physiol Regul Integr Comp Physiol. 2015 Nov 15;309(10):R1193-203 - PubMed
  3. J Biol Chem. 2001 Oct 26;276(43):39562-8 - PubMed
  4. FASEB J. 2002 May;16(7):706-8 - PubMed
  5. FEBS Lett. 2005 Jul 4;579(17):3601-6 - PubMed
  6. J Biol Chem. 1981 Apr 25;256(8):4007-16 - PubMed
  7. Trends Endocrinol Metab. 2006 Oct;17(8):301-7 - PubMed
  8. Science. 1992 Jan 17;255(5042):306-12 - PubMed
  9. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4766-71 - PubMed
  10. Cancer Res. 2007 Nov 15;67(22):11045-53 - PubMed
  11. J Mol Endocrinol. 2014 Apr 28;52(3):279-87 - PubMed
  12. FEBS Lett. 2000 Dec 15;486(3):247-51 - PubMed
  13. Nat Methods. 2012 Jul;9(7):671-5 - PubMed
  14. Mol Endocrinol. 2004 Oct;18(10):2522-42 - PubMed
  15. J Cell Sci. 2006 Oct 1;119(Pt 19):3901-3 - PubMed
  16. EMBO J. 2000 Mar 15;19(6):1187-94 - PubMed
  17. Endocrinology. 1992 Mar;130(3):1380-6 - PubMed
  18. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1246-51 - PubMed
  19. Nat Med. 2014 Jul;20(7):741-7 - PubMed
  20. Endocrinology. 1993 Aug;133(2):935-8 - PubMed
  21. Mol Endocrinol. 2005 Jul;19(7):1932-42 - PubMed
  22. Mol Endocrinol. 2003 Sep;17 (9):1815-23 - PubMed
  23. Invest Ophthalmol Vis Sci. 2001 Oct;42(11):2517-24 - PubMed
  24. Proc Natl Acad Sci U S A. 2001 Jan 30;98 (3):1024-9 - PubMed

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