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Mecatti GC, Fernandes Messias MC, Sant'Anna Paiola RM, et al. Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates. Biomark Insights. 2018;13:1177271918765137doi: 10.1177/1177271918765137.
Mecatti, G. C., Fernandes Messias, M. C., Sant'Anna Paiola, R. M., Figueiredo Angolini, C. F., da Silva Cunha, I. B., Eberlin, M. N., & de Oliveira Carvalho, P. (2018). Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates. Biomarker insights, 131177271918765137. https://doi.org/10.1177/1177271918765137
Mecatti, Giovana Colozza, et al. "Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates." Biomarker insights vol. 13 (2018): 1177271918765137. doi: https://doi.org/10.1177/1177271918765137
Mecatti GC, Fernandes Messias MC, Sant'Anna Paiola RM, Figueiredo Angolini CF, da Silva Cunha IB, Eberlin MN, de Oliveira Carvalho P. Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates. Biomark Insights. 2018 Mar 26;13:1177271918765137. doi: 10.1177/1177271918765137. eCollection 2018. PMID: 29623000; PMCID: PMC5882049.
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Biomark Insights. 2018 Mar 26;13:1177271918765137. doi: 10.1177/1177271918765137. eCollection 2018.

Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates.

Biomarker insights

Giovana Colozza Mecatti, Marcia Cristina Fernandes Messias, Rafaela Maria Sant'Anna Paiola, Célio Fernando Figueiredo Angolini, Ildenize Barbosa da Silva Cunha, Marcos Nogueira Eberlin, Patricia de Oliveira Carvalho

Affiliations

  1. Laboratory of Multidisciplinary Research, Sao Francisco University, USF, Sao Francisco de Assis Avenue, Brazil.
  2. Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, Brazil.

PMID: 29623000 PMCID: PMC5882049 DOI: 10.1177/1177271918765137

Abstract

BACKGROUND: Sepsis remains the primary cause of death from infection, despite advances in modern medicine. The identification of reliable diagnostic biomarkers for the early detection of this disease is critical and may reduce the mortality rate as it could allow early treatment. The purpose of this study was to describe the changes in the plasma and red cells blood lipidome profiling of patients diagnosed with sepsis and septic shock with the aim to identify potentially useful metabolic markers.

METHODS: Lipids from plasma and erythrocytes from septic patients (n = 20) and healthy controls (n = 20) were evaluated by electrospray ionization quadrupole time-of-flight mass spectrometry, and the fatty acid composition of the phospholipids fraction of erythrocytes was determined by gas chromatography. The data were treated with multivariate data analysis, including principal component analysis and (orthogonal) partial least squares discriminant analysis.

RESULTS: Potential biomarkers including lysophosphatidylcholines (lyso-PCs) and sphingomyelin (SMs) with specific fatty acid chains were identified. Both Lyso-PCs and SMs were downregulated, whereas the saturated and unsaturated phosphatidylcholines (PCs) were upregulated in the plasma and erythrocytes of septic patients. An increase in oleic acid (C18:1

CONCLUSIONS: These results suggest that lipidome profiling has great potential in discovering potential clinical biomarkers for sepsis and helping to understand its underlying mechanisms.

Keywords: Biomarkers; lipidomic; lysophosphatidylcholine; sepsis; sphingomyelin

Conflict of interest statement

Declaration of conflicting interests:The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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