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Rijal G, Bathula C, Li W. Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models. Int J Biomater. 2017;2017:8074890doi: 10.1155/2017/8074890.
Rijal, G., Bathula, C., & Li, W. (2017). Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models. International journal of biomaterials, 20178074890. https://doi.org/10.1155/2017/8074890
Rijal, Girdhari, et al. "Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models." International journal of biomaterials vol. 2017 (2017): 8074890. doi: https://doi.org/10.1155/2017/8074890
Rijal G, Bathula C, Li W. Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models. Int J Biomater. 2017;2017:8074890. doi: 10.1155/2017/8074890. Epub 2017 Dec 17. PMID: 29599800; PMCID: PMC5828246.
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Int J Biomater. 2017;2017:8074890. doi: 10.1155/2017/8074890. Epub 2017 Dec 17.

Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models.

International journal of biomaterials

Girdhari Rijal, Chandra Bathula, Weimin Li

Affiliations

  1. Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99210, USA.

PMID: 29599800 PMCID: PMC5828246 DOI: 10.1155/2017/8074890

Abstract

Preparation of three-dimensional (3D) porous scaffolds from synthetic polymers is a challenge to most laboratories conducting biomedical research. Here, we present a handy and cost-effective method to fabricate polymeric hydrogel and porous scaffolds using poly(lactic-co-glycolic) acid (PLGA) or polycaprolactone (PCL). Breast cancer cells grown on 3D polymeric scaffolds exhibited distinct survival, morphology, and proliferation compared to those on 2D polymeric surfaces. Mammary epithelial cells cultured on PLGA- or PCL-coated slides expressed extracellular matrix (ECM) proteins and their receptors. Estrogen receptor- (ER-) positive T47D breast cancer cells are less sensitive to 4-hydroxytamoxifen (4-HT) treatment when cultured on the 3D porous scaffolds than in 2D cultures. Finally, cancer cell-laden polymeric scaffolds support consistent tumor formation in animals and biomarker expression as seen in human native tumors. Our data suggest that the porous synthetic polymer scaffolds satisfy the basic requirements for 3D tissue cultures both

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