Display options
Cite
Eremin SA, Khan OY, Pisarev VV, et al. Fluorescence Polarization Immunoassay, for Express Control of Antibiotic Levels: Design and Characteristics for Chloramphenicol, as an Example. Antibiot Khimioter. 2016;61(9):22-27
Eremin, S. A., Khan, O. Y., Pisarev, V. V., Zvereva, E. A., Zherdev, A. V., & Dzantiev, B. B. (2016). Fluorescence Polarization Immunoassay, for Express Control of Antibiotic Levels: Design and Characteristics for Chloramphenicol, as an Example. Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic], 61(9), 22-27.
Eremin, S A, et al. "Fluorescence Polarization Immunoassay, for Express Control of Antibiotic Levels: Design and Characteristics for Chloramphenicol, as an Example." Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] vol. 61,9 (2016): 22-27.
Eremin SA, Khan OY, Pisarev VV, Zvereva EA, Zherdev AV, Dzantiev BB. Fluorescence Polarization Immunoassay, for Express Control of Antibiotic Levels: Design and Characteristics for Chloramphenicol, as an Example. Antibiot Khimioter. 2016;61(9):22-27. Russian. PMID: 29539247.
Copy Download .nbib Format: NLM
Share it on

Antibiot Khimioter. 2016;61(9):22-27.

Fluorescence Polarization Immunoassay, for Express Control of Antibiotic Levels: Design and Characteristics for Chloramphenicol, as an Example.

Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]

[Article in Russian]
S A Eremin, O Yu Khan, V V Pisarev, E A Zvereva, A V Zherdev, B B Dzantiev

PMID: 29539247

Abstract

Characteristics of the fluorescence polarization immunoassay (FPIA) as a mean for express control of antibiotic levels in various specimens and its advantages vs. other analytical tests are described. The developmental stages of the analytical procedure and its parameters are considered for chlorampnenicol as an example. The analysis is based on competitive interaction of anti-chloramphenicol antibodies with the chloramphenicol-fluorophore conjugate and the potential free chloramphenicol in the specimen. The experimental results of the comparison of the chloramphenicol FPIA with the use of two conjugates differing in the length of the bridge length between the antibiotic functional groups and fluorophore (fluorescein) are presented. The requirements to the choice of the antibody and conjugate concentrations providing highly sensitive detection are characterized. The detection limit of chloramphenicol in the FPIA was 10 ng/ml and the determination of the concentrations ranged from 20 ng/mI to 10 mcg/ml. The time of the assay was 10 min.

Publication Types