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Garcia-Prat M, Vila-Pijoan G, Martos Gutierrez S, et al. Age-specific pediatric reference ranges for immunoglobulins and complement proteins on the Optilite. J Clin Lab Anal. 2018;32(6):e22420doi: 10.1002/jcla.22420.
Garcia-Prat, M., Vila-Pijoan, G., Martos Gutierrez, S., Gala Yerga, G., García Guantes, E., Martínez-Gallo, M., Martín-Nalda, A., Soler-Palacín, P., & Hernández-González, M. (2018). Age-specific pediatric reference ranges for immunoglobulins and complement proteins on the Optilite. Journal of clinical laboratory analysis, 32(6), e22420. https://doi.org/10.1002/jcla.22420
Garcia-Prat, Marina, et al. "Age-specific pediatric reference ranges for immunoglobulins and complement proteins on the Optilite." Journal of clinical laboratory analysis vol. 32,6 (2018): e22420. doi: https://doi.org/10.1002/jcla.22420
Garcia-Prat M, Vila-Pijoan G, Martos Gutierrez S, Gala Yerga G, García Guantes E, Martínez-Gallo M, Martín-Nalda A, Soler-Palacín P, Hernández-González M. Age-specific pediatric reference ranges for immunoglobulins and complement proteins on the Optilite. J Clin Lab Anal. 2018 Jul;32(6):e22420. doi: 10.1002/jcla.22420. Epub 2018 Mar 30. PMID: 29603375; PMCID: PMC6817213.
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J Clin Lab Anal. 2018 Jul;32(6):e22420. doi: 10.1002/jcla.22420. Epub 2018 Mar 30.

Age-specific pediatric reference ranges for immunoglobulins and complement proteins on the Optilite.

Journal of clinical laboratory analysis

Marina Garcia-Prat, Gemma Vila-Pijoan, Susana Martos Gutierrez, Guadalupe Gala Yerga, Esther García Guantes, Mónica Martínez-Gallo, Andrea Martín-Nalda, Pere Soler-Palacín, Manuel Hernández-González

Affiliations

  1. Pediatric Infectious Diseases and Immunodeficiencies Unit (UPIIP), Hospital Universitari Vall d'Hebron (HUVH), Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
  2. Jeffrey Model Foundation Excellence Center, Barcelona, Spain.
  3. Immunology Division, Institut de Recerca Vall d'Hebron, Universitat Autònoma de Barcelona, Hospital Universitari, Vall d'Hebron, Barcelona, Spain.

PMID: 29603375 PMCID: PMC6817213 DOI: 10.1002/jcla.22420

Abstract

BACKGROUND: Measurement of immunoglobulins and complement proteins are frontline tests used in the assessment of immune system integrity, and reference values can vary with age. Their measurement provides an insight into the function of the innate and adaptive immune systems.

METHODS: We generated pediatric reference ranges for IgG, IgA, IgM, IgD, the IgG and IgA subclasses, and C3 and C4 using the Optilite™ turbidimetric analyzer.

RESULTS: The concentrations of IgG, IgA, and IgD showed an increase with age, as expected, while IgM remained stable between the age groups. For the IgG subclasses, no significant differences were observed in IgG1 or IgG3, while IgG2 and IgG4 concentrations increased steadily with age. The concentration of IgG2 plateaued at 15-18 years, while IgG4 plateaued at 10-14 years. The trend of concentrations across all groups was IgG1 > IgG2 > IgG3 > IgG4. For both IgA1 and IgA2, concentrations increased significantly with age, plateauing at 15-18 years. The median IgA1 concentration was greater than IgA2 across all groups. There was a good correlation between the total IgG or IgA concentration and summation of their subclasses (R

CONCLUSION: We have generated age-specific reference ranges in healthy children for C3, C4, IgG, IgA, IgM, IgD and the IgG and IgA subclasses using the Optilite

© 2018 Wiley Periodicals, Inc.

Keywords: complement system proteins; immunodeficiency; immunoglobulins; pediatric reference values; turbidimetry

References

  1. Mol Immunol. 1983 Sep;20(9):977-81 - PubMed
  2. Turk J Pediatr. 2006 Jan-Mar;48(1):19-24 - PubMed
  3. Nat Rev Immunol. 2017 Jan;17(1):21-29 - PubMed
  4. Curr Opin Allergy Clin Immunol. 2015 Dec;15(6):505-13 - PubMed
  5. Crit Rev Clin Lab Sci. 1983;18(3):213-60 - PubMed
  6. Clin Chem. 2003 Nov;49(11):1924-9 - PubMed
  7. Iran J Public Health. 2012;41(7):59-63 - PubMed
  8. J Rheumatol. 2002 Feb;29(2):288-91 - PubMed
  9. Clin Biochem. 2010 May;43(7-8):694-6 - PubMed
  10. Front Immunol. 2013 Feb 05;4:4 - PubMed
  11. Immunobiology. 2016 Nov;221(11):1247-58 - PubMed
  12. Clin Immunol. 2010 Dec;137(3):374-83 - PubMed
  13. Arch Dis Child. 1985 Mar;60(3):204-7 - PubMed
  14. J Pediatr (Rio J). 2011 Nov-Dec;87(6):512-6 - PubMed
  15. Annu Rev Immunol. 1993;11:501-38 - PubMed
  16. Arthritis Rheum. 1992 Jun;35(6):630-40 - PubMed
  17. Monogr Allergy. 1988;23:269-81 - PubMed
  18. Rheumatology (Oxford). 2016 Dec;55(suppl 2):ii23-ii29 - PubMed
  19. J Allergy Clin Immunol. 2015 Nov;136(5):1186-205.e1-78 - PubMed
  20. Br J Haematol. 2011 Jul;154(1):32-75 - PubMed
  21. Arthritis Res Ther. 2012 Jul 30;14(4):218 - PubMed
  22. Clin Chem. 1976 May;22(5):594-601 - PubMed
  23. Clin Exp Immunol. 2012 Jan;167(1):108-19 - PubMed
  24. Clin Diagn Lab Immunol. 1994 Nov;1(6):667-9 - PubMed
  25. Clin Exp Immunol. 1971 Sep;9(3):355-8 - PubMed
  26. Pediatr Res. 1979 Mar;13(3):181-3 - PubMed
  27. Immunopharmacology. 2000 Aug;49(1-2):187-98 - PubMed
  28. Clin Chem. 1982 Jan;28(1):126-8 - PubMed
  29. J Allergy Clin Immunol. 2010 Feb;125(2 Suppl 2):S41-52 - PubMed
  30. Pediatr Infect Dis J. 1990 Aug;9(8 Suppl):S9-15 - PubMed
  31. Acta Paediatr Scand. 1979 Jan;68(1):23-7 - PubMed
  32. J Exp Med. 1968 Mar 1;127(3):633-46 - PubMed
  33. Immunochemistry. 1970 Aug;7(8):703-8 - PubMed
  34. Annu Rev Immunol. 1986;4:231-51 - PubMed
  35. J Clin Pathol. 1983 Oct;36(10):1193-6 - PubMed
  36. Clin Exp Immunol. 2006 Aug;145(2):204-14 - PubMed
  37. Clin Exp Immunol. 1969 Jan;4(1):101-12 - PubMed
  38. Front Immunol. 2014 Sep 24;5:457 - PubMed
  39. J Clin Lab Anal. 2018 Jul;32(6):e22420 - PubMed
  40. Br J Haematol. 2009 Oct;147(1):22-42 - PubMed
  41. J Allergy Clin Immunol Pract. 2016 Jan-Feb;4(1):38-59 - PubMed
  42. Clin Chim Acta. 2003 Feb;328(1-2):129-33 - PubMed
  43. Curr Opin Pediatr. 1993 Dec;5(6):696-9 - PubMed
  44. Clin Chem Lab Med. 2001 Nov;39(11):1098-109 - PubMed
  45. Clin Chem Lab Med. 2015 Nov;53(12):e359-61 - PubMed
  46. Clin Exp Immunol. 1987 May;68(2):392-6 - PubMed
  47. Clin Biochem. 2009 Oct;42(15):1568-71 - PubMed

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