Display options
Cite
Jeong Y, You D, Kang HG, et al. Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells. J Breast Cancer. 2018;21(1):21-27doi: 10.4048/jbc.2018.21.1.21.
Jeong, Y., You, D., Kang, H. G., Yu, J., Kim, S. W., Nam, S. J., Lee, J. E., & Kim, S. (2018). Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells. Journal of breast cancer, 21(1), 21-27. https://doi.org/10.4048/jbc.2018.21.1.21
Jeong, Yisun, et al. "Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells." Journal of breast cancer vol. 21,1 (2018): 21-27. doi: https://doi.org/10.4048/jbc.2018.21.1.21
Jeong Y, You D, Kang HG, Yu J, Kim SW, Nam SJ, Lee JE, Kim S. Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells. J Breast Cancer. 2018 Mar;21(1):21-27. doi: 10.4048/jbc.2018.21.1.21. Epub 2018 Mar 23. PMID: 29628980; PMCID: PMC5880962.
Copy Download .nbib Format: NLM
Share it on

J Breast Cancer. 2018 Mar;21(1):21-27. doi: 10.4048/jbc.2018.21.1.21. Epub 2018 Mar 23.

Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells.

Journal of breast cancer

Yisun Jeong, Daeun You, Hyun-Gu Kang, Jonghan Yu, Seok Won Kim, Seok Jin Nam, Jeong Eon Lee, Sangmin Kim

Affiliations

  1. Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University, Seoul, Korea.
  2. Breast Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
  3. Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

PMID: 29628980 PMCID: PMC5880962 DOI: 10.4048/jbc.2018.21.1.21

Abstract

PURPOSE: The exact mechanism regulating fibronectin (FN) expression in breast cancer cells has not been fully elucidated. In this study, we investigated the pharmacological mechanism of berberine (BBR) with respect to FN expression in triple-negative breast cancer (TNBC) cells.

METHODS: The clinical significance of FN mRNA expression was analyzed using the Kaplan-Meier plotter database (http://kmplot.com/breast). FN mRNA and protein expression levels were analyzed by real-time polymerase chain reaction and western blotting, respectively.

RESULTS: Using publicly available clinical data, we observed that high FN expression was associated with poor prognosis in patients with breast cancer. FN mRNA and protein expression was increased in TNBC cells compared with non-TNBC cells. As expected, recombinant human FN significantly induced cell spreading and adhesion in MDA-MB231 TNBC cells. We also investigated the regulatory mechanism underlying FN expression. Basal levels of FN mRNA and protein expression were downregulated by a specific activator protein-1 (AP-1) inhibitor, SR11302. Interestingly, FN expression in TNBC cells was dose-dependently decreased by BBR treatment. The level of c-Jun phosphorylation was also decreased by BBR treatment.

CONCLUSION: Our findings demonstrate that FN expression is regulated via an AP-1-dependent mechanism, and that BBR suppresses FN expression in TNBC cells through inhibition of AP-1 activity.

Keywords: Berberine; Cell adhesion; Fibronectins; Transcription factor AP-1

Conflict of interest statement

CONFLICT OF INTEREST: The authors declare that they have no competing interests.

References

  1. Am J Med. 1984 Oct;77(4):685-9 - PubMed
  2. APMIS Suppl. 1992;26:1-39 - PubMed
  3. Arch Toxicol. 2007 Oct;81(10):719-28 - PubMed
  4. J Surg Res. 2012 Jul;176(1):e21-9 - PubMed
  5. Acta Biomater. 2014 Apr;10(4):1524-31 - PubMed
  6. Int J Oncol. 2007 Mar;30(3):621-9 - PubMed
  7. Br J Cancer. 2009 Jul 21;101(2):327-34 - PubMed
  8. Cell. 1992 Nov 13;71(4):671-8 - PubMed
  9. Cell Physiol Biochem. 2013;32(5):1541-50 - PubMed
  10. Cancer Metastasis Rev. 1995 Sep;14(3):173-89 - PubMed
  11. Biochem Pharmacol. 2006 Mar 14;71(6):806-17 - PubMed
  12. OMICS. 2013 Oct;17(10):510-8 - PubMed
  13. Planta Med. 1975 Dec;28(4):353-8 - PubMed
  14. Mech Ageing Dev. 1996 Jul 5;88(1-2):111-24 - PubMed
  15. Mutat Res. 2009 Mar 9;662(1-2):75-83 - PubMed
  16. Cancer Res. 2010 Jul 1;70(13):5238-48 - PubMed
  17. Cancer Lett. 2009 Jul 8;279(2):155-62 - PubMed
  18. Oncol Rep. 2014 Dec;32(6):2777-88 - PubMed
  19. Planta Med. 2008 Jan;74(1):39-42 - PubMed
  20. Biomacromolecules. 2008 Jul;9(7):1843-51 - PubMed
  21. J Cell Biol. 1991 Sep;114(6):1295-305 - PubMed
  22. J Cell Sci. 2011 Feb 1;124(Pt 3):369-83 - PubMed
  23. Exp Cell Res. 2015 Apr 10;333(1):116-26 - PubMed
  24. Eur J Pharmacol. 2014 Oct 5;740:584-95 - PubMed
  25. Nature. 2008 Jul 24;454(7203):436-44 - PubMed
  26. Carcinogenesis. 2006 Oct;27(10):2018-27 - PubMed
  27. J Biol Chem. 1998 Oct 9;273(41):26487-96 - PubMed
  28. Cancer. 1983 Mar 15;51(6):1142-7 - PubMed
  29. Clin Cancer Res. 2008 Sep 1;14(17):5555-64 - PubMed
  30. Breast Cancer Res Treat. 2010 Oct;123(3):725-31 - PubMed

Publication Types