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Noroozi N, Gargari SLM, Nazarian S, et al. Immunogenicity of enterotoxigenic . Iran J Basic Med Sci. 2018;21(3):284-291doi: 10.22038/ijbms.2018.25886.6371.
Noroozi, N., Gargari, S. L. M., Nazarian, S., Sarvary, S., & Adriani, R. R. (2018). Immunogenicity of enterotoxigenic . Iranian journal of basic medical sciences, 21(3), 284-291. https://doi.org/10.22038/ijbms.2018.25886.6371
Noroozi, Nafiseh, et al. "Immunogenicity of enterotoxigenic ." Iranian journal of basic medical sciences vol. 21,3 (2018): 284-291. doi: https://doi.org/10.22038/ijbms.2018.25886.6371
Noroozi N, Gargari SLM, Nazarian S, Sarvary S, Adriani RR. Immunogenicity of enterotoxigenic . Iran J Basic Med Sci. 2018 Mar;21(3):284-291. doi: 10.22038/ijbms.2018.25886.6371. PMID: 29511495; PMCID: PMC5817172.
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Iran J Basic Med Sci. 2018 Mar;21(3):284-291. doi: 10.22038/ijbms.2018.25886.6371.

Immunogenicity of enterotoxigenic .

Iranian journal of basic medical sciences

Nafiseh Noroozi, Seyed Latif Mousavi Gargari, Shahram Nazarian, Samaneh Sarvary, Razieh Rezaei Adriani

Affiliations

  1. Department of Biology, Faculty of Science, Shahed University, Tehran, Iran.
  2. Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran.

PMID: 29511495 PMCID: PMC5817172 DOI: 10.22038/ijbms.2018.25886.6371

Abstract

OBJECTIVES: Enterotoxigenic

MATERIALS AND METHODS: OMVs was purified from bacterial supernatant by ultracentrifugation. OMVs were encapsulated in chitosan nanoparticles prepared by ionic gelation method within a layer of Eudragit L100 for oral delivery. Female BALB/c mice of 9 weeks' old were immunized by parenteral injection and oral administration with free and encapsulated OMVs obtained from bacteria cultivated at 37°C and 42°C. The serum samples were collected and the antibody titers were measured by an enzyme-linked immunosorbent assay (ELISA).

RESULTS: The protein concentrations of OMVs were 3.47 mg/ml and 2.46 mg/ml for bacteria grown at 37°C and 42°C respectively. OMVs loaded into nanoparticles (NP-OMVs) were homogeneous and spherical in shape, with a size of 532 nm. The encapsulation efficiency of NP was 90%. Mice immunized with OMVs, inhibited the ETEC colonization in their small intestine and induced production of antibodies against LT toxin.

CONCLUSION: The results obtained in this research place OMVs among promising candidates to be used for vaccination.

Keywords: Chitosan nanoparticle; Enterotoxigenic –; Escherichia coli; Outer membrane vesicle; Virulence factors

References

  1. FEMS Immunol Med Microbiol. 2003 May 25;36(3):199-205 - PubMed
  2. Clin Microbiol Rev. 2005 Jul;18(3):465-83 - PubMed
  3. Infect Immun. 2010 Oct;78(10):4402-20 - PubMed
  4. Microbiology. 2014 Oct;160(Pt 10):2109-21 - PubMed
  5. Microbiol Res. 2014 Feb-Mar;169(2-3):205-12 - PubMed
  6. Jpn J Infect Dis. 2014;67(2):78-85 - PubMed
  7. Bioessays. 2007 Jun;29(6):591-604 - PubMed
  8. Vaccine. 2009 Jun 24;27 Suppl 2:B3-12 - PubMed
  9. Clin Vaccine Immunol. 2011 Nov;18(11):1803-8 - PubMed
  10. Annu Rev Microbiol. 2010;64:163-84 - PubMed
  11. Vaccine. 2007 Mar 30;25(14):2545-66 - PubMed
  12. Protein Cell. 2015 Jul;6(7):480-503 - PubMed
  13. Vaccine. 2014 Dec 12;32(52):7077-84 - PubMed
  14. J Travel Med. 2005 Mar-Apr;12(2):102-7 - PubMed
  15. Scand J Immunol. 2012 Aug;76(2):99-107 - PubMed
  16. Pediatr Infect Dis J. 2004 Dec;23(12 Suppl):S285-92 - PubMed
  17. J Infect Dis. 2002 Feb 15;185(4):497-502 - PubMed
  18. Iran Biomed J. 2014;18(4):212-218 - PubMed
  19. Front Microbiol. 2015 Aug 11;6:823 - PubMed
  20. Infect Immun. 2008 Oct;76(10):4554-63 - PubMed
  21. Adv Drug Deliv Rev. 2015 Aug 30;91:52-69 - PubMed
  22. PLoS Negl Trop Dis. 2013 Jun 27;7(6):e2293 - PubMed
  23. J Food Drug Anal. 2015 Dec;23 (4):619-629 - PubMed
  24. Vaccine. 2010 Jul 5;28(30):4810-6 - PubMed
  25. J Bacteriol. 2013 Jul;195(13):2971-81 - PubMed
  26. Curr Opin Immunol. 2005 Aug;17(4):388-98 - PubMed
  27. Infect Immun. 2008 May;76(5):2106-12 - PubMed
  28. Infect Immun. 2010 Jul;78(7):3027-35 - PubMed
  29. Eur J Pharm Biopharm. 2012 May;81(1):43-8 - PubMed
  30. J Microbiol Methods. 2012 Jul;90(1):36-45 - PubMed
  31. Microbiol Res. 2015 Jan;170:1-9 - PubMed
  32. Braz J Infect Dis. 2014 May-Jun;18(3):308-14 - PubMed
  33. Vaccine. 2013 Jul 11;31(32):3288-94 - PubMed
  34. Adv Drug Deliv Rev. 2012 May 1;64(6):557-70 - PubMed
  35. Vaccine. 2003 Jan 17;21(5-6):341-6 - PubMed
  36. Front Immunol. 2014 Mar 24;5:121 - PubMed

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