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Molinaro R, Evangelopoulos M, Hoffman JR, et al. Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach. Adv Mater. 2018;30(15):e1702749doi: 10.1002/adma.201702749.
Molinaro, R., Evangelopoulos, M., Hoffman, J. R., Corbo, C., Taraballi, F., Martinez, J. O., Hartman, K. A., Cosco, D., Costa, G., Romeo, I., Sherman, M., Paolino, D., Alcaro, S., & Tasciotti, E. (2018). Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach. Advanced materials (Deerfield Beach, Fla.), 30(15), e1702749. https://doi.org/10.1002/adma.201702749
Molinaro, Roberto, et al. "Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach." Advanced materials (Deerfield Beach, Fla.) vol. 30,15 (2018): e1702749. doi: https://doi.org/10.1002/adma.201702749
Molinaro R, Evangelopoulos M, Hoffman JR, Corbo C, Taraballi F, Martinez JO, Hartman KA, Cosco D, Costa G, Romeo I, Sherman M, Paolino D, Alcaro S, Tasciotti E. Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach. Adv Mater. 2018 Apr;30(15):e1702749. doi: 10.1002/adma.201702749. Epub 2018 Mar 07. PMID: 29512198.
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Adv Mater. 2018 Apr;30(15):e1702749. doi: 10.1002/adma.201702749. Epub 2018 Mar 07.

Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach.

Advanced materials (Deerfield Beach, Fla.)

Roberto Molinaro, Michael Evangelopoulos, Jessica R Hoffman, Claudia Corbo, Francesca Taraballi, Jonathan O Martinez, Kelly A Hartman, Donato Cosco, Giosue' Costa, Isabella Romeo, Michael Sherman, Donatella Paolino, Stefano Alcaro, Ennio Tasciotti

Affiliations

  1. Center of Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.
  2. Nanoinspired Biomedicine Lab, Fondazione Istituto di Ricerca, Pediatrica Città della Speranza, 35127, Padua, Italy.
  3. Department of Health Sciences, University "Magna Graecia" of Catanzaro, Campus Universitario "S. Venuta,", Viale S. Venuta, Germaneto, I-88100, Catanzaro, Italy.
  4. Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, 77555, USA.
  5. Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Campus Universitario "S. Venuta,", Viale S. Venuta, Germaneto, I-88100, Catanzaro, Italy.
  6. Houston Methodist Orthopedic and Sports Medicine, Houston Methodist Hospital, 6565 Fannin Street, Houston, TX, 77030, USA.

PMID: 29512198 DOI: 10.1002/adma.201702749

Abstract

The advancement of nanotechnology toward more sophisticated bioinspired approaches has highlighted the gap between the advantages of biomimetic and biohybrid platforms and the availability of manufacturing processes to scale up their production. Though the advantages of transferring biological features from cells to synthetic nanoparticles for drug delivery purposes have recently been reported, a standardizable, batch-to-batch consistent, scalable, and high-throughput assembly method is required to further develop these platforms. Microfluidics has offered a robust tool for the controlled synthesis of nanoparticles in a versatile and reproducible approach. In this study, the incorporation of membrane proteins within the bilayer of biomimetic nanovesicles (leukosomes) using a microfluidic-based platform is demonstrated. The physical, pharmaceutical, and biological properties of microfluidic-formulated leukosomes (called NA-Leuko) are characterized. NA-Leuko show extended shelf life and retention of the biological functions of donor cells (i.e., macrophage avoidance and targeting of inflamed vasculature). The NA approach represents a universal, versatile, robust, and scalable tool, which is extensively used for the assembly of lipid nanoparticles and adapted here for the manufacturing of biomimetic nanovesicles.

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: bioinspired approach; inflammation; membrane protein incorporation; microfluidics; molecular dynamics

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