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Gu J, Wu W, Huang M, et al. Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules. 2018;23(4)doi: 10.3390/molecules23040884.
Gu, J., Wu, W., Huang, M., Long, F., Liu, X., & Zhu, Y. (2018). Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules (Basel, Switzerland), 23(4), . https://doi.org/10.3390/molecules23040884
Gu, Jifeng, et al. "Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements." Molecules (Basel, Switzerland) vol. 23,4 (2018). doi: https://doi.org/10.3390/molecules23040884
Gu J, Wu W, Huang M, Long F, Liu X, Zhu Y. Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules. 2018 Apr 11;23(4). doi: 10.3390/molecules23040884. PMID: 29641474; PMCID: PMC6017834.
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Molecules. 2018 Apr 11;23(4). doi: 10.3390/molecules23040884.

Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements.

Molecules (Basel, Switzerland)

Jifeng Gu, Weijun Wu, Mengwei Huang, Fen Long, Xinhua Liu, Yizhun Zhu

Affiliations

  1. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  2. Department of pharmacy, Eye & ENT Hospital of Fudan University, Shanghai 200031, China. [email protected].
  3. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  4. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  5. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  6. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  7. Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. [email protected].
  8. State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau, China. [email protected].

PMID: 29641474 PMCID: PMC6017834 DOI: 10.3390/molecules23040884

Abstract

A method for high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap high-resolution mass spectrometry (HPLC-LTQ-Orbitrap MS) was developed and validated for the qualitative and quantitative assessment of Shejin-liyan Granule. According to the fragmentation mechanism and high-resolution MS data, 54 compounds, including fourteen isoflavones, eleven ligands, eight flavonoids, six physalins, six organic acids, four triterpenoid saponins, two xanthones, two alkaloids, and one licorice coumarin, were identified or tentatively characterized. In addition, ten of the representative compounds (matrine, galuteolin, tectoridin, iridin, arctiin, tectorigenin, glycyrrhizic acid, irigenin, arctigenin, and irisflorentin) were quantified using the validated HPLC-LTQ-Orbitrap MS method. The method validation showed a good linearity with coefficients of determination (r²) above 0.9914 for all analytes. The accuracy of the intra- and inter-day variation of the investigated compounds was 95.0-105.0%, and the precision values were less than 4.89%. The mean recoveries and reproducibilities of each analyte were 95.1-104.8%, with relative standard deviations below 4.91%. The method successfully quantified the ten compounds in Shejin-liyan Granule, and the results show that the method is accurate, sensitive, and reliable.

Keywords: HPLC-LTQ-Orbitrap MS; Shejin-liyan Granule; identification; quality assessment

Conflict of interest statement

The authors declare no conflict of interest.

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