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Keshavarzi S, Salehi M, Farifteh-Nobijari F, et al. Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes. Cell J. 2018;20(2):244-249doi: 10.22074/cellj.2018.4860.
Keshavarzi, S., Salehi, M., Farifteh-Nobijari, F., Hosseini, T., Hosseini, S., Ghazifard, A., Ghaffari Novin, M., Fallah-Omrani, V., Nourozian, M., & Hosseini, A. (2018). Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes. Cell journal, 20(2), 244-249. https://doi.org/10.22074/cellj.2018.4860
Keshavarzi, Somayeh, et al. "Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes." Cell journal vol. 20,2 (2018): 244-249. doi: https://doi.org/10.22074/cellj.2018.4860
Keshavarzi S, Salehi M, Farifteh-Nobijari F, Hosseini T, Hosseini S, Ghazifard A, Ghaffari Novin M, Fallah-Omrani V, Nourozian M, Hosseini A. Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes. Cell J. 2018 Jul;20(2):244-249. doi: 10.22074/cellj.2018.4860. Epub 2018 Mar 18. PMID: 29633602; PMCID: PMC5893296.
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Cell J. 2018 Jul;20(2):244-249. doi: 10.22074/cellj.2018.4860. Epub 2018 Mar 18.

Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes.

Cell journal

Somayeh Keshavarzi, Mohammad Salehi, Fattaneh Farifteh-Nobijari, Taher Hosseini, Sara Hosseini, Alaleh Ghazifard, Marefat Ghaffari Novin, Vahid Fallah-Omrani, Mohsen Nourozian, Ahmad Hosseini

Affiliations

  1. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  2. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  3. Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  4. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Electronic address:[email protected].

PMID: 29633602 PMCID: PMC5893296 DOI: 10.22074/cellj.2018.4860

Abstract

OBJECTIVES: We evaluated the effect of melatonin, as a potent antioxidant agent, on glutathione (GSH) and reactive oxygen species (ROS) levels, as well as histone H3 lysine 9 (H3K9), and H4 lysine 12 (H4K12) acetylation when added to oocytes culture medium.

MATERIALS AND METHODS: In this experimental study, two in vitro and in vivo groups were used. In the in vitro group, cumulus oocyte complexes (COCs) from the ovaries of B6D2F1 mice were cultured in maturation medium containing two doses of melatonin (10

RESULTS: The H4K12 acetylation and ROS levels were significantly higher in the oocytes matured in the in vitro group compared to the in vivo group (P<0.05). Furthermore, glutathione levels in the in vitro group were considerably lower than that of the in vivo group (P<0.05). Melatonin at the concentration of 10

CONCLUSIONS: Exogenous melatonin improves the competence of mouse oocytes during in vitro maturation (IVM).

Copyright© by Royan Institute. All rights reserved.

Keywords: Glutathione; In Vitro Oocyte Maturation; Melatonin; Reactive Oxygen Species

Conflict of interest statement

There is no conflict of interest in this study.

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