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Muguregowda HT, Kumar P, Govindarama PUE. Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study. World J Plast Surg. 2018;7(1):58-66
Muguregowda, H. T., Kumar, P., & Govindarama, P. U. E. (2018). Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study. World journal of plastic surgery, 7(1), 58-66.
Muguregowda, Honnegowda Thittamaranahalli, et al. "Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study." World journal of plastic surgery vol. 7,1 (2018): 58-66.
Muguregowda HT, Kumar P, Govindarama PUE. Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study. World J Plast Surg. 2018 Jan;7(1):58-66. PMID: 29651393; PMCID: PMC5890367.
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World J Plast Surg. 2018 Jan;7(1):58-66.

Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study.

World journal of plastic surgery

Honnegowda Thittamaranahalli Muguregowda, Pramod Kumar, Padmanabha Udupa Echalasara Govindarama

Affiliations

  1. Department of Plastic Surgery and Burns, Kasturba Medical College, Manipal, Karnataka, India.
  2. Department of Plastic Surgery and Burns, King Abdul Aziz Specialist Hospital, Sakaka, Al-jouf Saudi Arabia.
  3. Department of Biochemistry, Kasturba Medical College, Manipal, Karnataka, India.

PMID: 29651393 PMCID: PMC5890367

Abstract

BACKGROUND: Malondialdehyde (MDA) is an oxidant that causes damage to membranes, DNA, proteins, and lipids at the cellular level. Antioxidants minimize the effects of oxidants and thus help in formation of healthy granulation tissues with higher level of hydroxyproline and total protein. This study compared the effect of limited access dressing (LAD) with conventional closed dressing biochemically and histopathologically.

METHODS: Seventy-two 12-65 years old burn patients with mean wound size of 14 cm

RESULTS: LAD group showed significant increase in hydroxyproline, total protein, GSH, and GPx and decrease in MDA levels compared to conventional dressing group. A significant negative correlation between GSH and MDA was noted in LAD group, but in conventional dressing group there was no significant correlation. A significant negative correlation between GPx and MDA was noticed in LAD group, but in conventional dressing group was not significant. There was a histologically fewer inflammatory cells, increased and well organized extracellular matrix deposit, more angiogenesis in LAD group after 10 days while the difference was significant between the groups.

CONCLUSION: Our study showed a significant reduction in oxidative stress biomarker of MDA, increase in hydroxyproline, total protein, antioxidants and amount of ECM deposition, number of blood vessels and a decrease in the amount of inflammatory cells and necrotic tissues in LAD group indicating the better healing effect of burn wounds.

Keywords: Antioxidant; Burn; Wound; Limited access dressing; Malondialdehyde; ROS

Conflict of interest statement

The authors declare no conflict of interest.

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